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Open AccessArticle

Marker Development for Differentiation of Fusarium oxysporum f. sp. Niveum Race 3 from Races 1 and 2

1
Department of Plant Pathology, University of Georgia, Tifton, GA 31793, USA
2
Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA
3
Department of Plant and Environmental Sciences, Clemson University, Charleston, SC 29414, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(2), 822; https://doi.org/10.3390/ijms22020822
Received: 2 December 2020 / Revised: 8 January 2021 / Accepted: 11 January 2021 / Published: 15 January 2021
(This article belongs to the Section Molecular Microbiology)
Fusarium wilt of watermelon, caused by Fusarium oxysporum f. sp. niveum (FON), is pathogenic only to watermelon and has become one of the main limiting factors in watermelon production internationally. Detection methods for this pathogen are limited, with few published molecular assays available to differentiate FON from other formae speciales of F. oxysporum. FON has four known races that vary in virulence but are difficult and costly to differentiate using traditional inoculation methods and only race 2 can be differentiated molecularly. In this study, genomic and chromosomal comparisons facilitated the development of a conventional polymerase chain reaction (PCR) assay that could differentiate race 3 from races 1 and 2, and by using two other published PCR markers in unison with the new marker, the three races could be differentiated. The new PCR marker, FNR3-F/FNR3-R, amplified a 511 bp region on the “pathogenicity chromosome” of the FON genome that is absent in race 3. FNR3-F/FNR3-R detected genomic DNA down to 2.0 pg/µL. This marker, along with two previously published FON markers, was successfully applied to test over 160 pathogenic FON isolates from Florida, Georgia, and South Carolina. Together, these three FON primer sets worked well for differentiating races 1, 2, and 3 of FON. For each marker, a greater proportion (60 to 90%) of molecular results agreed with the traditional bioassay method of race differentiation compared to those that did not. The new PCR marker should be useful to differentiate FON races and improve Fusarium wilt research. View Full-Text
Keywords: Fusarium oxysporum f. sp. niveum; comparative genomics; race differentiation; polymerase chain reaction; watermelon Fusarium oxysporum f. sp. niveum; comparative genomics; race differentiation; polymerase chain reaction; watermelon
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MDPI and ACS Style

Hudson, O.; Waliullah, S.; Fulton, J.C.; Ji, P.; Dufault, N.S.; Keinath, A.; Ali, M.E. Marker Development for Differentiation of Fusarium oxysporum f. sp. Niveum Race 3 from Races 1 and 2. Int. J. Mol. Sci. 2021, 22, 822. https://doi.org/10.3390/ijms22020822

AMA Style

Hudson O, Waliullah S, Fulton JC, Ji P, Dufault NS, Keinath A, Ali ME. Marker Development for Differentiation of Fusarium oxysporum f. sp. Niveum Race 3 from Races 1 and 2. International Journal of Molecular Sciences. 2021; 22(2):822. https://doi.org/10.3390/ijms22020822

Chicago/Turabian Style

Hudson, Owen; Waliullah, Sumyya; Fulton, James C.; Ji, Pingsheng; Dufault, Nicholas S.; Keinath, Anthony; Ali, Md E. 2021. "Marker Development for Differentiation of Fusarium oxysporum f. sp. Niveum Race 3 from Races 1 and 2" Int. J. Mol. Sci. 22, no. 2: 822. https://doi.org/10.3390/ijms22020822

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