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Article

Effect of Polyhexamethylene Biguanide in Combination with Undecylenamidopropyl Betaine or PslG on Biofilm Clearance

by 1,2,†, 1,† and 1,3,*
1
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
2
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
3
Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China
*
Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Int. J. Mol. Sci. 2021, 22(2), 768; https://doi.org/10.3390/ijms22020768
Received: 10 December 2020 / Revised: 5 January 2021 / Accepted: 8 January 2021 / Published: 14 January 2021
(This article belongs to the Special Issue Microbial Biofilms and Antibiofilm Agents)
Hospital-acquired infection is a great challenge for clinical treatment due to pathogens’ biofilm formation and their antibiotic resistance. Here, we investigate the effect of antiseptic agent polyhexamethylene biguanide (PHMB) and undecylenamidopropyl betaine (UB) against biofilms of four pathogens that are often found in hospitals, including Gram-negative bacteria Pseudomonas aeruginosa and Escherichia coli, Gram-positive bacteria Staphylococcus aureus, and pathogenic fungus, Candida albicans. We show that 0.02% PHMB, which is 10-fold lower than the concentration of commercial products, has a strong inhibitory effect on the growth, initial attachment, and biofilm formation of all tested pathogens. PHMB can also disrupt the preformed biofilms of these pathogens. In contrast, 0.1% UB exhibits a mild inhibitory effect on biofilm formation of the four pathogens. This concentration inhibits the growth of S. aureus and C. albicans yet has no growth effect on P. aeruginosa or E. coli. UB only slightly enhances the anti-biofilm efficacy of PHMB on P. aeruginosa biofilms. However, pretreatment with PslG, a glycosyl hydrolase that can efficiently inhibit and disrupt P. aeruginosa biofilm, highly enhances the clearance effect of PHMB on P. aeruginosa biofilms. Meanwhile, PslG can also disassemble the preformed biofilms of the other three pathogens within 30 min to a similar extent as UB treatment for 24 h. View Full-Text
Keywords: biofilm; polyhexamethylene biguanide (PHMB); undecylenamidopropyl betaine (UB); PslG biofilm; polyhexamethylene biguanide (PHMB); undecylenamidopropyl betaine (UB); PslG
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MDPI and ACS Style

Zheng, Y.; Wang, D.; Ma, L.Z. Effect of Polyhexamethylene Biguanide in Combination with Undecylenamidopropyl Betaine or PslG on Biofilm Clearance. Int. J. Mol. Sci. 2021, 22, 768. https://doi.org/10.3390/ijms22020768

AMA Style

Zheng Y, Wang D, Ma LZ. Effect of Polyhexamethylene Biguanide in Combination with Undecylenamidopropyl Betaine or PslG on Biofilm Clearance. International Journal of Molecular Sciences. 2021; 22(2):768. https://doi.org/10.3390/ijms22020768

Chicago/Turabian Style

Zheng, Yaqian, Di Wang, and Luyan Z. Ma 2021. "Effect of Polyhexamethylene Biguanide in Combination with Undecylenamidopropyl Betaine or PslG on Biofilm Clearance" International Journal of Molecular Sciences 22, no. 2: 768. https://doi.org/10.3390/ijms22020768

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