Next Article in Journal
Novel Nested-Seq Approach for SARS-CoV-2 Real-Time Epidemiology and In-Depth Mutational Profiling in Wastewater
Next Article in Special Issue
The Bul1/2 Alpha-Arrestins Promote Ubiquitylation and Endocytosis of the Can1 Permease upon Cycloheximide-Induced TORC1-Hyperactivation
Previous Article in Journal
Mitochondrial Carriers and Substrates Transport Network: A Lesson from Saccharomyces cerevisiae
Previous Article in Special Issue
Heterodimer Formation of the Homodimeric ABC Transporter OpuA
Article

Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat

1
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, China
2
Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China
*
Author to whom correspondence should be addressed.
Contributed equally to this paper.
Academic Editors: Stathis Frillingos and Maria Botou
Int. J. Mol. Sci. 2021, 22(16), 8497; https://doi.org/10.3390/ijms22168497
Received: 18 July 2021 / Revised: 3 August 2021 / Accepted: 4 August 2021 / Published: 7 August 2021
(This article belongs to the Special Issue Transport Proteins for Microbial Adaptations 2.0)
Wheat is a major staple food crop worldwide, due to its total yield and unique processing quality. Its grain yield and quality are threatened by Fusarium head blight (FHB), which is mainly caused by Fusarium graminearum. Salicylic acid (SA) has a strong and toxic effect on F. graminearum and is a hopeful target for sustainable control of FHB. F. graminearum is capable of efficientdealing with SA stress. However, the underlying mechanisms remain unclear. Here, we characterized FgMFS1 (FGSG_03725), a major facilitator superfamily (MFS) transporter gene in F. graminearum. FgMFS1 was highly expressed during infection and was upregulated by SA. The predicted three-dimensional structure of the FgMFS1 protein was consistent with the schematic for the antiporter. The subcellular localization experiment indicated that FgMFS1 was usually expressed in the vacuole of hyphae, but was alternatively distributed in the cell membrane under SA treatment, indicating an element of F. graminearum in response to SA. ΔFgMFS1 (loss of function mutant of FgMFS1) showed enhanced sensitivity to SA, less pathogenicity towards wheat, and reduced DON production under SA stress. Re-introduction of a functional FgMFS1 gene into ∆FgMFS1 recovered the mutant phenotypes. Wheat spikes inoculated with ΔFgMFS1 accumulated more SA when compared to those inoculated with the wild-type strain. Ecotopic expression of FgMFS1 in yeast enhanced its tolerance to SA as expected, further demonstrating that FgMFS1 functions as an SA exporter. In conclusion, FgMFS1 encodes an SA exporter in F. graminearum, which is critical for its response to wheat endogenous SA and pathogenicity towards wheat. View Full-Text
Keywords: MFS transporter; fusarium head blight; phytohormone; sustainable disease control MFS transporter; fusarium head blight; phytohormone; sustainable disease control
Show Figures

Figure 1

MDPI and ACS Style

Chen, Q.; Lei, L.; Liu, C.; Zhang, Y.; Xu, Q.; Zhu, J.; Guo, Z.; Wang, Y.; Li, Q.; Li, Y.; Kong, L.; Jiang, Y.; Lan, X.; Wang, J.; Jiang, Q.; Chen, G.; Ma, J.; Wei, Y.; Zheng, Y.; Qi, P. Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat. Int. J. Mol. Sci. 2021, 22, 8497. https://doi.org/10.3390/ijms22168497

AMA Style

Chen Q, Lei L, Liu C, Zhang Y, Xu Q, Zhu J, Guo Z, Wang Y, Li Q, Li Y, Kong L, Jiang Y, Lan X, Wang J, Jiang Q, Chen G, Ma J, Wei Y, Zheng Y, Qi P. Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat. International Journal of Molecular Sciences. 2021; 22(16):8497. https://doi.org/10.3390/ijms22168497

Chicago/Turabian Style

Chen, Qing, Lu Lei, Caihong Liu, Yazhou Zhang, Qiang Xu, Jing Zhu, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Li Kong, Yunfeng Jiang, Xiujin Lan, Jirui Wang, Qiantao Jiang, Guoyue Chen, Jian Ma, Yuming Wei, Youliang Zheng, and Pengfei Qi. 2021. "Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat" International Journal of Molecular Sciences 22, no. 16: 8497. https://doi.org/10.3390/ijms22168497

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop