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Article

A Novel Allele Encoding 7-Hydroxymethyl Chlorophyll a Reductase Confers Bacterial Blight Resistance in Rice

1
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
2
Lianyungang Academy of Agricultural Sciences, Lianyungang 222006, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Pedro Martínez-Gómez
Int. J. Mol. Sci. 2021, 22(14), 7585; https://doi.org/10.3390/ijms22147585
Received: 23 June 2021 / Revised: 12 July 2021 / Accepted: 13 July 2021 / Published: 15 July 2021
(This article belongs to the Collection Genetics and Molecular Breeding in Plants)
Rice spotted leaf mutants are helpful to investigate programmed cell death (PCD) and defense response pathways in plants. Using a map-based cloning strategy, we characterized novel rice spotted leaf mutation splHM143 that encodes a 7-hydroxymethyl chlorophyll a reductase (OsHCAR). The wild-type (WT) allele could rescue the mutant phenotype, as evidenced by complementation analysis. OsHCAR was constitutively expressed at all rice tissues tested and its expression products localized to chloroplasts. The mutant exhibited PCD and leaf senescence with increased H2O2 (hydrogen peroxide) accumulation, increased of ROS (reactive oxygen species) scavenging enzymes activities and TUNEL (terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling) -positive nuclei, upregulation of PCD related genes, decreased chlorophyll (Chl) contents, downregulation of photosynthesis-related genes, and upregulation of senescence-associated genes. Besides, the mutant exhibited enhanced bacterial blight resistance with significant upregulation of defense response genes. Knockout lines of OsHCAR exhibited spotted leaf phenotype, cell death, leaf senescence, and showed increased resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) coupled with upregulation of five pathogenesis-related marker genes. The overexpression of OsHCAR resulted in increased susceptibility to Xoo with decreased expression of pathogenesis-related marker genes. Altogether, our findings revealed that OsHCAR is involved in regulating cell death and defense response against bacterial blight pathogen in rice. View Full-Text
Keywords: rice; bacterial blight; defense response; OsHCAR rice; bacterial blight; defense response; OsHCAR
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MDPI and ACS Style

Kampire, M.G.; Sanglou, R.K.; Wang, H.; Kazeem, B.B.; Wu, J.-l.; Zhang, X. A Novel Allele Encoding 7-Hydroxymethyl Chlorophyll a Reductase Confers Bacterial Blight Resistance in Rice. Int. J. Mol. Sci. 2021, 22, 7585. https://doi.org/10.3390/ijms22147585

AMA Style

Kampire MG, Sanglou RK, Wang H, Kazeem BB, Wu J-l, Zhang X. A Novel Allele Encoding 7-Hydroxymethyl Chlorophyll a Reductase Confers Bacterial Blight Resistance in Rice. International Journal of Molecular Sciences. 2021; 22(14):7585. https://doi.org/10.3390/ijms22147585

Chicago/Turabian Style

Kampire, Marie Gorette, Ringki Kuinamei Sanglou, Huimei Wang, Bello Babatunde Kazeem, Jian-li Wu, and Xiaobo Zhang. 2021. "A Novel Allele Encoding 7-Hydroxymethyl Chlorophyll a Reductase Confers Bacterial Blight Resistance in Rice" International Journal of Molecular Sciences 22, no. 14: 7585. https://doi.org/10.3390/ijms22147585

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