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Open AccessArticle

A Tetra-Panel of Serum Circulating miRNAs for the Diagnosis of the Four Most Prevalent Tumor Types

1
Laboratory of Molecular Biology, Fundación Instituto Valenciano de Oncología, 46009 Valencia, Spain
2
IVO-CIPF Joint Research Unit of Cancer, Príncipe Felipe Research Center (CIPF), 46012 Valencia, Spain
3
Department of Medical Oncology, Fundación Instituto Valenciano de Oncología, 46009 Valencia, Spain
4
Unit of Gastroenterology, Fundación Instituto Valenciano de Oncología, 46009 Valencia, Spain
5
Department of Thoracic Surgery, Fundación Instituto Valenciano de Oncología, 46009 Valencia, Spain
6
Department of Radiology, Fundación Instituto Valenciano de Oncología, 46009 Valencia, Spain
7
Department of Urology, Fundación Instituto Valenciano de Oncología, 46009 Valencia, Spain
8
Nanophotonics Technology Center, Universitat Politècnica de València, 46022 Valencia, Spain
9
Department of Pathology, School of Medicine, Catholic University of Valencia ‘San Vicente Mártir’, 46001 Valencia, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(8), 2783; https://doi.org/10.3390/ijms21082783
Received: 17 March 2020 / Revised: 9 April 2020 / Accepted: 15 April 2020 / Published: 16 April 2020
(This article belongs to the Special Issue MicroRNA Biology in Cancer Development)
The purpose of this study is to clinically validate a series of circulating miRNAs that distinguish between the 4 most prevalent tumor types (lung cancer (LC); breast cancer (BC); colorectal cancer (CRC); and prostate cancer (PCa)) and healthy donors (HDs). A total of 18 miRNAs and 3 housekeeping miRNA genes were evaluated by qRT-PCR on RNA extracted from serum of cancer patients, 44 LC, 45 BC, 27 CRC, and 40 PCa, and on 45 HDs. The cancer detection performance of the miRNA expression levels was evaluated by studying the area under the curve (AUC) of receiver operating characteristic (ROC) curves at univariate and multivariate levels. miR-21 was significantly overexpressed in all cancer types compared with HDs, with accuracy of 67.5% (p = 0.001) for all 4 tumor types and of 80.8% (p < 0.0001) when PCa cases were removed from the analysis. For each tumor type, a panel of miRNAs was defined that provided cancer-detection accuracies of 91%, 94%, 89%, and 77%, respectively. In conclusion, we have described a series of circulating miRNAs that define different tumor types with a very high diagnostic performance. These panels of miRNAs would constitute the basis of different approaches of cancer-detection systems for which clinical utility should be validated in prospective cohorts. View Full-Text
Keywords: Early diagnosis; circulating microRNAs; lung cancer; breast cancer; colorectal cancer; prostate cancer Early diagnosis; circulating microRNAs; lung cancer; breast cancer; colorectal cancer; prostate cancer
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Pastor-Navarro, B.; García-Flores, M.; Fernández-Serra, A.; Blanch-Tormo, S.; Martínez de Juan, F.; Martínez-Lapiedra, C.; Maia de Alcantara, F.; Peñalver, J.C.; Cervera-Deval, J.; Rubio-Briones, J.; García-Rupérez, J.; López-Guerrero, J.A. A Tetra-Panel of Serum Circulating miRNAs for the Diagnosis of the Four Most Prevalent Tumor Types. Int. J. Mol. Sci. 2020, 21, 2783.

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