Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent
by
Mateusz Szymczak 1
, Bartłomiej Grygorcewicz 2, Joanna Karczewska-Golec 3, Przemysław Decewicz 3, Jarosław Adam Pankowski 1, Hanna Országh-Szturo 1, Paweł Bącal 4,5, Barbara Dołęgowska 2 and Piotr Golec 1,*
Mateusz Szymczak 1, Bartłomiej Grygorcewicz 2, Joanna Karczewska-Golec 3, Przemysław Decewicz 3, Jarosław Adam Pankowski 1, Hanna Országh-Szturo 1, Paweł Bącal 4,5, Barbara Dołęgowska 2 and Piotr Golec 1,*
1
Department of Molecular Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
2
Department of Laboratory Medicine, Chair of Microbiology, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland
3
Department of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
4
Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Ksiecia Trojdena 4, 02-109 Warsaw, Poland
5
Laboratory of Theory and Applications of Electrodes, Faculty of Chemistry, University of Warsaw, 02-093 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(4), 1403; https://doi.org/10.3390/ijms21041403
Received: 26 January 2020 / Revised: 12 February 2020 / Accepted: 17 February 2020 / Published: 19 February 2020
(This article belongs to the Special Issue Bacteriophage—Molecular Studies)
Bordetella bronchiseptica, an emerging zoonotic pathogen, infects a broad range of mammalian hosts. B. bronchiseptica-associated atrophic rhinitis incurs substantial losses to the pig breeding industry. The true burden of human disease caused by B. bronchiseptica is unknown, but it has been postulated that some hypervirulent B. bronchiseptica isolates may be responsible for undiagnosed respiratory infections in humans. B. bronchiseptica was shown to acquire antibiotic resistance genes from other bacterial genera, especially Escherichia coli. Here, we present a new B. bronchiseptica lytic bacteriophage—vB_BbrP_BB8—of the Podoviridae family, which offers a safe alternative to antibiotic treatment of B. bronchiseptica infections. We explored the phage at the level of genome, physiology, morphology, and infection kinetics. Its therapeutic potential was investigated in biofilms and in an in vivo Galleria mellonella model, both of which mimic the natural environment of infection. The BB8 is a unique phage with a genome structure resembling that of T7-like phages. Its latent period is 75 ± 5 min and its burst size is 88 ± 10 phages. The BB8 infection causes complete lysis of B. bronchiseptica cultures irrespective of the MOI used. The phage efficiently removes bacterial biofilm and prevents the lethality induced by B. bronchiseptica in G. mellonella honeycomb moth larvae.
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Keywords:
phage therapy; zoonosis; emerging diseases; antibiotic resistance; Galleria mellonella; phage stability; biofilm; animal model; atrophic rhinitis; veterinary microbiology
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MDPI and ACS Style
Szymczak, M.; Grygorcewicz, B.; Karczewska-Golec, J.; Decewicz, P.; Pankowski, J.A.; Országh-Szturo, H.; Bącal, P.; Dołęgowska, B.; Golec, P. Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent. Int. J. Mol. Sci. 2020, 21, 1403.
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