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Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2

Department of Functioning of Living Systems, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia
Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), 17198 Moscow, Russia
Faculty of Biology and Biotechnology, National Research University Higher School of Economics, 101000 Moscow, Russia
N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia
E.O. Mukhin Municipal Clinical Hospital, 111399 Moscow, Russia
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(23), 9167;
Received: 6 November 2020 / Revised: 28 November 2020 / Accepted: 28 November 2020 / Published: 1 December 2020
(This article belongs to the Special Issue COVID-19 and Molecular Studies in Biology and Chemistry)
In 2020 the world faced the pandemic of COVID-19 severe acute respiratory syndrome caused by a new type of coronavirus named SARS-CoV-2. To stop the spread of the disease, it is crucial to create molecular tools allowing the investigation, diagnoses and treatment of COVID-19. One of such tools are monoclonal antibodies (mAbs). In this study we describe the development of hybridoma cells that can produce mouse mAbs against receptor binding domain of SARS-CoV-2 spike (S) protein. These mAbs are able to specifically detect native and denatured S proteins in all tested applications, including immunoblotting, enzyme-linked immunosorbent assay, immunofluorescence staining of cells and immunohistochemical staining of paraffin embedded patients’ tissue samples. In addition, we showed that the obtained mAbs can efficiently block SARS-CoV-2 infection in in vitro experiments. Finally, we determined the amino acid sequence of light and heavy chains of the mAbs. This information will allow the use of corresponding peptides to establish genetically engineered therapeutic antibodies. To date multiple mAbs against SARS-CoV-2 proteins have been established, however, bigger sets of various antibodies will allow the detection and neutralization of SARS-CoV-2, even if the virus acquires novel mutations. View Full-Text
Keywords: SARS-CoV-2; Spike RBD; COVID19; hybridoma; monoclonal antibodies SARS-CoV-2; Spike RBD; COVID19; hybridoma; monoclonal antibodies
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MDPI and ACS Style

Antipova, N.V.; Larionova, T.D.; Siniavin, A.E.; Nikiforova, M.A.; Gushchin, V.A.; Babichenko, I.I.; Volkov, A.V.; Shakhparonov, M.I.; Pavlyukov, M.S. Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2. Int. J. Mol. Sci. 2020, 21, 9167.

AMA Style

Antipova NV, Larionova TD, Siniavin AE, Nikiforova MA, Gushchin VA, Babichenko II, Volkov AV, Shakhparonov MI, Pavlyukov MS. Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2. International Journal of Molecular Sciences. 2020; 21(23):9167.

Chicago/Turabian Style

Antipova, Nadezhda V., Tatyana D. Larionova, Andrei E. Siniavin, Maria A. Nikiforova, Vladimir A. Gushchin, Igor I. Babichenko, Alexey V. Volkov, Michail I. Shakhparonov, and Marat S. Pavlyukov 2020. "Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2" International Journal of Molecular Sciences 21, no. 23: 9167.

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