Next Article in Journal
Combination Therapy to Treat Fungal Biofilm-Based Infections
Next Article in Special Issue
Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives
Previous Article in Journal
TBP-Related Factor 2 as a Trigger for Robertsonian Translocations and Speciation
Previous Article in Special Issue
Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis
Article

Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes

1
Oxidative Stress and Plant Proteomics Group, Institute of Plant Science and Microbiology, Universität Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany
2
Department of Ecogenomics and Systems Biology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria
3
Center for Diagnostics Clinical Chemistry and Laboratory Medicine, Core Facility of Mass Spectrometric Proteomic, Campus Forschung N27, University Hospital Hamburg-Eppendorf (UKE), Martinistrasse 52, 20246 Hamburg, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8872; https://doi.org/10.3390/ijms21228872
Received: 14 October 2020 / Revised: 19 November 2020 / Accepted: 20 November 2020 / Published: 23 November 2020
(This article belongs to the Special Issue Plant Proteomic Research 3.0)
Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production and scavenging enzymes in plants. The influence of hypoxia on roots of hydroponically grown maize (Zea mays L.) plants was investigated. Gene expression (RNA Seq and RT-qPCR) and proteome (LC–MS/MS and 2D-PAGE) analyses were used to determine the alterations in soluble and membrane-bound class III peroxidases under hypoxia. Gel-free peroxidase analyses of plasma membrane-bound proteins showed an increased abundance of ZmPrx03, ZmPrx24, ZmPrx81, and ZmPr85 in stressed samples. Furthermore, RT-qPCR analyses of the corresponding peroxidase genes revealed an increased expression. These peroxidases could be separated with 2D-PAGE and identified by mass spectrometry. An increased abundance of ZmPrx03 and ZmPrx85 was determined. Further peroxidases were identified in detergent-insoluble membranes. Co-regulation with a respiratory burst oxidase homolog (Rboh) and key enzymes of the phenylpropanoid pathway indicates a function of the peroxidases in membrane protection, aerenchyma formation, and cell wall remodeling under hypoxia. This hypothesis was supported by the following: (i) an elevated level of hydrogen peroxide and aerenchyma formation; (ii) an increased guaiacol peroxidase activity in membrane fractions of stressed samples, whereas a decrease was observed in soluble fractions; and (iii) alterations in lignified cells, cellulose, and suberin in root cross-sections. View Full-Text
Keywords: aerenchyma; cell wall remodeling; class III peroxidases; hypoxia; maize roots; plasma membrane; respiratory burst oxidase homolog; Zea mays L. aerenchyma; cell wall remodeling; class III peroxidases; hypoxia; maize roots; plasma membrane; respiratory burst oxidase homolog; Zea mays L.
Show Figures

Graphical abstract

MDPI and ACS Style

Hofmann, A.; Wienkoop, S.; Harder, S.; Bartlog, F.; Lüthje, S. Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes. Int. J. Mol. Sci. 2020, 21, 8872. https://doi.org/10.3390/ijms21228872

AMA Style

Hofmann A, Wienkoop S, Harder S, Bartlog F, Lüthje S. Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes. International Journal of Molecular Sciences. 2020; 21(22):8872. https://doi.org/10.3390/ijms21228872

Chicago/Turabian Style

Hofmann, Anne, Stefanie Wienkoop, Sönke Harder, Fabian Bartlog, and Sabine Lüthje. 2020. "Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes" International Journal of Molecular Sciences 21, no. 22: 8872. https://doi.org/10.3390/ijms21228872

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop