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Open AccessArticle

An Injectable Hyaluronan–Methylcellulose (HAMC) Hydrogel Combined with Wharton’s Jelly-Derived Mesenchymal Stromal Cells (WJ-MSCs) Promotes Degenerative Disc Repair

1
Department of Neurosurgery, CHA Bundang Medical Center, CHA University School of medicine, Seongnam-si 13496, Korea
2
Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA
3
Department of Neurosurgery, School of Medicine, Kyungpook National University, Daegu 41944, Korea
4
Department of Neurosurgery, Kyungpook National University Hospital, Daegu 41404, Korea
5
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON M5S 3E5, Canada
6
Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(19), 7391; https://doi.org/10.3390/ijms21197391
Received: 5 September 2020 / Revised: 25 September 2020 / Accepted: 2 October 2020 / Published: 7 October 2020
Intervertebral disc (IVD) degeneration is one of the predominant causes of chronic low back pain (LBP), which is a leading cause of disability worldwide. Despite substantial progress in cell therapy for the treatment of IVD degeneration, significant challenges remain for clinical application. Here, we investigated the effectiveness of hyaluronan–methylcellulose (HAMC) hydrogels loaded with Wharton’s Jelly-derived mesenchymal stromal cell (WJ-MSCs) in vitro and in a rat coccygeal IVD degeneration model. Following induction of injury-induced IVD degeneration, female Sprague-Dawley rats were randomized into four groups to undergo a single intradiscal injection of the following: (1) phosphate buffered saline (PBS) vehicle, (2) HAMC, (3) WJ-MSCs (2 × 104 cells), and (4) WJ-MSCs-loaded HAMC (WJ-MSCs/HAMC) (n = 10/each group). Coccygeal discs were removed following sacrifice 6 weeks after implantation for radiologic and histologic analysis. We confirmed previous findings that encapsulation in HAMC increases the viability of WJ-MSCs for disc repair. The HAMC gel maintained significant cell viability in vitro. In addition, combined implantation of WJ-MSCs and HAMC significantly promoted degenerative disc repair compared to WJ-MSCs alone, presumably by improving nucleus pulposus cells viability and decreasing extracellular matrix degradation. Our results suggest that WJ-MSCs-loaded HAMC promotes IVD repair more effectively than cell injection alone and supports the potential clinical use of HAMC for cell delivery to arrest IVD degeneration or to promote IVD regeneration. View Full-Text
Keywords: mesenchymal stromal cell; Wharton jelly; hyaluronic acid; methylcellulose; intervertebral disc degeneration; regeneration; extracellular matrix mesenchymal stromal cell; Wharton jelly; hyaluronic acid; methylcellulose; intervertebral disc degeneration; regeneration; extracellular matrix
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Choi, U.Y.; Joshi, H.P.; Payne, S.; Kim, K.T.; Kyung, J.W.; Choi, H.; Cooke, M.J.; Kwon, S.Y.; Roh, E.J.; Sohn, S.; Shoichet, M.S.; Han, I. An Injectable Hyaluronan–Methylcellulose (HAMC) Hydrogel Combined with Wharton’s Jelly-Derived Mesenchymal Stromal Cells (WJ-MSCs) Promotes Degenerative Disc Repair. Int. J. Mol. Sci. 2020, 21, 7391.

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