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Open AccessArticle

Physiological Importance of Pectin Modifying Genes During Rice Pollen Development

1
Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea
2
Department of Plant & Environmental New Resources, College of Life Sciences, Kyung Hee University, Yongin 17104, Korea
3
School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(14), 4840; https://doi.org/10.3390/ijms21144840
Received: 23 May 2020 / Revised: 30 June 2020 / Accepted: 7 July 2020 / Published: 8 July 2020
(This article belongs to the Section Molecular Plant Sciences)
Although cell wall dynamics, particularly modification of homogalacturonan (HGA, a major component of pectin) during pollen tube growth, have been extensively studied in dicot plants, little is known about how modification of the pollen tube cell wall regulates growth in monocot plants. In this study, we assessed the role of HGA modification during elongation of the rice pollen tube by adding a pectin methylesterase (PME) enzyme or a PME-inhibiting catechin extract (Polyphenon 60) to in vitro germination medium. Both treatments led to a severe decrease in the pollen germination rate and elongation. Furthermore, using monoclonal antibodies toward methyl-esterified and de-esterified HGA epitopes, it was found that exogenous treatment of PME and Polyphenon 60 resulted in the disruption of the distribution patterns of low- and high-methylesterified pectins upon pollen germination and during pollen tube elongation. Eleven PMEs and 13 PME inhibitors (PMEIs) were identified by publicly available transcriptome datasets and their specific expression was validated by qRT-PCR. Enzyme activity assays and subcellular localization using a heterologous expression system in tobacco leaves demonstrated that some of the pollen-specific PMEs and PMEIs possessed distinct enzymatic activities and targeted either the cell wall or other compartments. Taken together, our findings are the first line of evidence showing the essentiality of HGA methyl-esterification status during the germination and elongation of pollen tubes in rice, which is primarily governed by the fine-tuning of PME and PMEI activities. View Full-Text
Keywords: rice; pollen; pectin; pectin methylesterase; pectin methylesterase inhibitor; pollen tube growth rice; pollen; pectin; pectin methylesterase; pectin methylesterase inhibitor; pollen tube growth
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    Doi: 10.5281/zenodo.3840891
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    Description: Table S1. Rice transcriptome data used in the study for genome-wide identification of pollen specific genes Table S2. Primers used for qRT-PCR Table S3. Primers used to isolate full-length cDNA for protein localization and enzyme activity Table S4. Major features of rice pollen specific PMEs and PMEIs Figure S1. Domain structure of group 1 PMEs studied in the study. Note the presence of at least one of cleavage site in the N-terminal. Four basic motifs (putative cleavage sites: RRLL, RRML, KLDL and RKLL) of the consensus sequence were found in rice group-1 PMEs
MDPI and ACS Style

Kim, Y.-J.; Jeong, H.Y.; Kang, S.-Y.; Silva, J.; Kim, E.-J.; Park, S.K.; Jung, K.-H.; Lee, C. Physiological Importance of Pectin Modifying Genes During Rice Pollen Development. Int. J. Mol. Sci. 2020, 21, 4840. https://doi.org/10.3390/ijms21144840

AMA Style

Kim Y-J, Jeong HY, Kang S-Y, Silva J, Kim E-J, Park SK, Jung K-H, Lee C. Physiological Importance of Pectin Modifying Genes During Rice Pollen Development. International Journal of Molecular Sciences. 2020; 21(14):4840. https://doi.org/10.3390/ijms21144840

Chicago/Turabian Style

Kim, Yu-Jin; Jeong, Ho Y.; Kang, Seung-Yeon; Silva, Jeniffer; Kim, Eui-Jung; Park, Soon K.; Jung, Ki-Hong; Lee, Chanhui. 2020. "Physiological Importance of Pectin Modifying Genes During Rice Pollen Development" Int. J. Mol. Sci. 21, no. 14: 4840. https://doi.org/10.3390/ijms21144840

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