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Open AccessArticle

Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani

1
Department of General and Medical Biochemistry, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland
2
Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Abrahama 58, 80-307 Gdańsk, Poland
3
Department of Environmental Toxicology, Faculty of Health Sciences with Institute of Maritime and Tropical Medicine, Medical University of Gdańsk, Dębowa 23A, 80-204 Gdańsk, Poland
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(10), 3687; https://doi.org/10.3390/ijms21103687
Received: 10 April 2020 / Revised: 19 May 2020 / Accepted: 20 May 2020 / Published: 23 May 2020
(This article belongs to the Special Issue Microbial Virulence Factors)
The Lon protein is a protease implicated in the virulence of many pathogenic bacteria, including some plant pathogens. However, little is known about the role of Lon in bacteria from genus Dickeya. This group of bacteria includes important potato pathogens, with the most aggressive species, D. solani. To determine the importance of Lon for pathogenicity and response to stress conditions of bacteria, we constructed a D. solani Δlon strain. The mutant bacteria showed increased sensitivity to certain stress conditions, in particular osmotic and high-temperature stresses. Furthermore, qPCR analysis showed an increased expression of the lon gene in D. solani under these conditions. The deletion of the lon gene resulted in decreased motility, lower activity of secreted pectinolytic enzymes and finally delayed onset of blackleg symptoms in the potato plants. In the Δlon cells, the altered levels of several proteins, including virulence factors and proteins associated with virulence, were detected by means of Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) analysis. These included components of the type III secretion system and proteins involved in bacterial motility. Our results indicate that Lon protease is important for D. solani to withstand stressful conditions and effectively invade the potato plant. View Full-Text
Keywords: protease Lon; Dickeya solani; plant pathogen; virulence factors; motility; type III secretion system; pathogenicity; resistance to stress; lon expression; pectinolytic enzymes protease Lon; Dickeya solani; plant pathogen; virulence factors; motility; type III secretion system; pathogenicity; resistance to stress; lon expression; pectinolytic enzymes
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MDPI and ACS Style

Figaj, D.; Czaplewska, P.; Przepióra, T.; Ambroziak, P.; Potrykus, M.; Skorko-Glonek, J. Lon Protease Is Important for Growth under Stressful Conditions and Pathogenicity of the Phytopathogen, Bacterium Dickeya solani. Int. J. Mol. Sci. 2020, 21, 3687.

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