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Article

The Impact of Glycosylation of Osteopontin on Urinary Stone Formation

1
Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan
2
Department of Urology, Tohoku Medical and Pharmaceutical University, Sendai 983-8536, Japan
3
Department of Advanced Transplant and Regenerative Medicine, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan
4
Department of Cancer Immunology and Cell Biology, Oyokyo Kidney Research Institute, Hirosaki, Aomori 036-8243, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 93; https://doi.org/10.3390/ijms21010093
Received: 11 November 2019 / Revised: 17 December 2019 / Accepted: 19 December 2019 / Published: 21 December 2019
(This article belongs to the Section Molecular Biology)
Osteopontin (OPN) is a matrix glycoprotein of urinary calculi. This study aims to identify the role of aberrant glycosylation of OPN in urolithiasis. We retrospectively measured urinary glycosylated OPN normalized by urinary full-length-OPN levels in 110 urolithiasis patients and 157 healthy volunteers and 21 patients were prospectively longitudinal follow-up during stone treatment. The urinary full-length-OPN levels were measured using enzyme-linked immunosorbent assay and glycosylated OPN was measured using a lectin array and lectin blotting. The assays were evaluated using the area under the receiver operating characteristics curve to discriminate stone forming urolithiasis patients. In the retrospective cohort, urinary Gal3C-S lectin reactive- (Gal3C-S-) OPN/full-length-OPN, was significantly higher in the stone forming urolithiasis patients than in the healthy volunteers (p < 0.0001), with good discrimination (AUC, 0.953), 90% sensitivity, and 92% specificity. The Lycopersicon esculentum lectin analysis of urinary full-length-OPN showed that urinary full-length-OPN in stone forming urolithiasis patients had a polyLacNAc structure that was not observed in healthy volunteers. In the prospective longitudinal follow-up study, 92.8% of the stone-free urolithiasis group had Gal3C-S-OPN/full-length-OPN levels below the cutoff value after ureteroscopic lithotripsy (URS), whereas 71.4% of the residual-stone urolithiasis group did not show decreased levels after URS. Therefore, Gal3C-S-OPN/full-length-OPN levels could be used as a urolithiasis biomarker. View Full-Text
Keywords: osteopontin; aberrant glycosylation; urolithiasis biomarker osteopontin; aberrant glycosylation; urolithiasis biomarker
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MDPI and ACS Style

Anan, G.; Yoneyama, T.; Noro, D.; Tobisawa, Y.; Hatakeyama, S.; Sutoh Yoneyama, M.; Yamamoto, H.; Imai, A.; Iwamura, H.; Kohada, Y.; Mikami, J.; Ito, J.; Kaiho, Y.; Yoneyama, T.; Hashimoto, Y.; Sato, M.; Ohyama, C. The Impact of Glycosylation of Osteopontin on Urinary Stone Formation. Int. J. Mol. Sci. 2020, 21, 93. https://doi.org/10.3390/ijms21010093

AMA Style

Anan G, Yoneyama T, Noro D, Tobisawa Y, Hatakeyama S, Sutoh Yoneyama M, Yamamoto H, Imai A, Iwamura H, Kohada Y, Mikami J, Ito J, Kaiho Y, Yoneyama T, Hashimoto Y, Sato M, Ohyama C. The Impact of Glycosylation of Osteopontin on Urinary Stone Formation. International Journal of Molecular Sciences. 2020; 21(1):93. https://doi.org/10.3390/ijms21010093

Chicago/Turabian Style

Anan, Go, Tohru Yoneyama, Daisuke Noro, Yuki Tobisawa, Shingo Hatakeyama, Mihoko Sutoh Yoneyama, Hayato Yamamoto, Atsushi Imai, Hiromichi Iwamura, Yuki Kohada, Jotaro Mikami, Jun Ito, Yasuhiro Kaiho, Takahiro Yoneyama, Yasuhiro Hashimoto, Makoto Sato, and Chikara Ohyama. 2020. "The Impact of Glycosylation of Osteopontin on Urinary Stone Formation" International Journal of Molecular Sciences 21, no. 1: 93. https://doi.org/10.3390/ijms21010093

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