Next Article in Journal
Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray
Next Article in Special Issue
Role of Macrophages in Cardioprotection
Previous Article in Journal
Novel Therapeutic Potentials of Taxifolin for Amyloid-β-associated Neurodegenerative Diseases and Other Diseases: Recent Advances and Future Perspectives
Previous Article in Special Issue
Use of Multifactorial Treatments to Address the Challenge of Translating Experimental Myocardial Infarct Reduction Strategies
Open AccessArticle

Effect of Ischemic Preconditioning and Postconditioning on Exosome-Rich Fraction microRNA Levels, in Relation with Electrophysiological Parameters and Ventricular Arrhythmia in Experimental Closed-Chest Reperfused Myocardial Infarction

1
Department of Internal Medicine II, Division of Cardiology, Medical University of Vienna, 1090 Vienna, Austria
2
Department of Pharmacology and Pharmacotherapy, Semmelweis University, 1089 Budapest, Hungary
3
Pharmahungary Group, 6722 Szeged, Hungary
4
National Heart Centre Singapore, Singapore 169609, Singapore
5
Cardiovascular and Metabolic Disorders Program, Duke-National University of Singapore, Singapore 169857, Singapore
6
The Hatter Cardiovascular Institute, University College London, London WC1E 6HX, UK
7
Barts Heart Centre, St Bartholomew’s Hospital, London EC1A 7BE, UK
8
The National Institute of Health Research University College London Hospitals Biomedical Research Centre, London W1T 7DN, UK
9
Yong Loo Lin School of Medicine, National University Singapore, Singapore 117597, Singapore
10
Institute for Regenerative Medicine (IREM), University of Zurich, 8952 Zurich, Switzerland
11
Department of Cardiovascular Surgery, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany
12
Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin, 13353 Berlin, Germany
13
Center for MR-Research, University Children’s Hospital Zurich, 8032 Zürich, Switzerland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(9), 2140; https://doi.org/10.3390/ijms20092140
Received: 2 April 2019 / Revised: 24 April 2019 / Accepted: 28 April 2019 / Published: 30 April 2019
We investigated the antiarrhythmic effects of ischemic preconditioning (IPC) and postconditioning (PostC) by intracardiac electrocardiogram (ECG) and measured circulating microRNAs (miRs) that are related to cardiac conduction. Domestic pigs underwent 90-min. percutaneous occlusion of the mid left anterior coronary artery, followed by reperfusion. The animals were divided into three groups: acute myocardial infarction (AMI, n = 7), ischemic preconditioning-acute myocardial infarction (IPC-AMI) (n = 9), or AMI-PostC (n = 5). IPC was induced by three 5-min. episodes of repetitive ischemia/reperfusion cycles (rI/R) before AMI. PostC was induced by six 30-s rI/R immediately after induction of reperfusion 90 min after occlusion. Before the angiographic procedure, a NOGA endocardial mapping catheter was placed again the distal anterior ventricular endocardium to record the intracardiac electrogram (R-amplitude, ST-Elevation, ST-area under the curve (AUC), QRS width, and corrected QT time (QTc)) during the entire procedure. An arrhythmia score was calculated. Cardiac MRI was performed after one-month. IPC led to significantly lower ST-elevation, heart rate, and arrhythmia score during ischemia. PostC induced a rapid recovery of R-amplitude, decrease in QTc, and lower arrhythmia score during reperfusion. Slightly higher levels of miR-26 and miR-133 were observed in AMI compared to groups IPC-AMI and AMI-PostC. Significantly lower levels of miR-1, miR-208, and miR-328 were measured in the AMI-PostC group as compared to animals in group AMI and IPC-AMI. The arrhythmia score was not significantly associated with miRNA plasma levels. Cardiac MRI showed significantly smaller infarct size in the IPC-AMI group when compared to the AMI and AMI-PostC groups. Thus, IPC led to better left ventricular ejection fraction at one-month and it exerted antiarrhythmic effects during ischemia, whereas PostC exhibited antiarrhythmic properties after reperfusion, with significant downregulaton of ischemia-related miRNAs. View Full-Text
Keywords: miRNA; extracellular vesicles; exosomes; exosome-rich fraction; ischemic preconditioning; ischemic postconditioning; acute myocardial infarction; cardiac electrophysiology; electrocardiography; ventricular arrhythmia; reperfusion arrhythmia; ischemia-reperfusion injury miRNA; extracellular vesicles; exosomes; exosome-rich fraction; ischemic preconditioning; ischemic postconditioning; acute myocardial infarction; cardiac electrophysiology; electrocardiography; ventricular arrhythmia; reperfusion arrhythmia; ischemia-reperfusion injury
Show Figures

Figure 1

MDPI and ACS Style

Spannbauer, A.; Traxler, D.; Lukovic, D.; Zlabinger, K.; Winkler, J.; Gugerell, A.; Ferdinandy, P.; Hausenloy, D.J.; Pavo, N.; Emmert, M.Y.; Hoerstrup, S.P.; Jakab, A.; Gyöngyösi, M.; Riesenhuber, M. Effect of Ischemic Preconditioning and Postconditioning on Exosome-Rich Fraction microRNA Levels, in Relation with Electrophysiological Parameters and Ventricular Arrhythmia in Experimental Closed-Chest Reperfused Myocardial Infarction. Int. J. Mol. Sci. 2019, 20, 2140.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop