Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis

Ondee, Thunnicha; Jaroonwitchawan, Thiranut; Pisitkun, Trairak; Gillen, Joseph; Nita-Lazar, Aleksandra; Leelahavanichkul, Asada; Somparn, Poorichaya

doi:10.3390/ijms20061354

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Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis

Thunnicha Ondee

¹,

Thiranut Jaroonwitchawan

²,

Trairak Pisitkun

Joseph Gillen

⁴,

Aleksandra Nita-Lazar

⁴

Asada Leelahavanichkul

^1,2,* and

Poorichaya Somparn

^3,*

Medical Sciences Program, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand

Center of Excellence in Immunology and Immune-mediated Diseases, Department of Microbiology, Chulalongkorn University, Bangkok 10330, Thailand

Center of Excellence in Systems Biology, Research affairs, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand

⁴

Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1892, USA

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2019, 20(6), 1354; https://doi.org/10.3390/ijms20061354

Received: 13 February 2019 / Revised: 13 March 2019 / Accepted: 14 March 2019 / Published: 18 March 2019

(This article belongs to the Special Issue Systemic Lupus Erythematosus: Research Updates in Immunopathogenesis, Biomarkers and Therapeutics)

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Abstract

Dysfunction of FcGRIIb, the only inhibitory receptor of the FcGR family, is commonly found in the Asian population and is possibly responsible for the extreme endotoxin exhaustion in lupus. Here, the mechanisms of prominent endotoxin (LPS) tolerance in FcGRIIb−/− mice were explored on bone marrow-derived macrophages using phosphoproteomic analysis. As such, LPS tolerance decreased several phosphoproteins in the FcGRIIb−/− macrophage, including protein kinase C-β type II (PRKCB), which was associated with phagocytosis function. Overexpression of PRKCB attenuated LPS tolerance in RAW264.7 cells, supporting the role of this gene in LPS tolerance. In parallel, LPS tolerance in macrophages and in mice was attenuated by phorbol 12-myristate 13-acetate (PMA) administration. This treatment induced several protein kinase C families, including PRKCB. However, PMA attenuated the severity of mice with cecal ligation and puncture on LPS tolerance preconditioning in FcGRIIb−/− but not in wild-type cells. The significant reduction of PRKCB in the FcGRIIb−/− macrophage over wild-type cell possibly induced the more severe LPS-exhaustion and increased the infection susceptibility in FcGRIIb−/− mice. PMA induced PRKCB, improved LPS-tolerance, and attenuated sepsis severity, predominantly in FcGRIIb−/− mice. PRKCB enhancement might be a promising strategy to improve macrophage functions in lupus patients with LPS-tolerance from chronic infection. View Full-Text

Keywords: intestinal candida; dextran sulfate solution induced colitis; dysbiosis; bacteremia; gut leakage

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Ondee, T.; Jaroonwitchawan, T.; Pisitkun, T.; Gillen, J.; Nita-Lazar, A.; Leelahavanichkul, A.; Somparn, P. Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis. Int. J. Mol. Sci. 2019, 20, 1354. https://doi.org/10.3390/ijms20061354

AMA Style

Ondee T, Jaroonwitchawan T, Pisitkun T, Gillen J, Nita-Lazar A, Leelahavanichkul A, Somparn P. Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis. International Journal of Molecular Sciences. 2019; 20(6):1354. https://doi.org/10.3390/ijms20061354

Chicago/Turabian Style

Ondee, Thunnicha, Thiranut Jaroonwitchawan, Trairak Pisitkun, Joseph Gillen, Aleksandra Nita-Lazar, Asada Leelahavanichkul, and Poorichaya Somparn. 2019. "Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis" International Journal of Molecular Sciences 20, no. 6: 1354. https://doi.org/10.3390/ijms20061354

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Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis

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