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Article

Involvement of Receptor for Advanced Glycation Endproducts in Hypertensive Disorders of Pregnancy

1
Department of Obstetrics and Gynecology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan
2
Department of Biochemistry, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(21), 5462; https://doi.org/10.3390/ijms20215462
Received: 29 August 2019 / Revised: 18 October 2019 / Accepted: 28 October 2019 / Published: 1 November 2019
(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Preeclampsia)
Preeclampsia/hypertensive disorders of pregnancy (PE/HDP) is a serious and potentially life-threatening disease. Recently, PE/HDP has been considered to cause adipose tissue inflammation, but the detailed mechanism remains unknown. We exposed human primary cultured adipocytes with serum from PE/HDP and healthy controls for 24 h, and analyzed mRNA expression of several adipokines, cytokines, and ligands of the receptor for advanced glycation endproducts (RAGE). We found that the mRNA levels of interleukin-6 (IL-6), C-C motif chemokine ligand 2 (CCL2), high mobility group box 1 (HMGB1), and RAGE were significantly increased by the addition of PE/HDP serum. Among RAGE ligands, advanced glycation endproducts (AGE) and HMGB1 increased mRNA levels of IL-6 and CCL2 in SW872 human adipocytes and mouse 3T3-L1 cells. The introduction of small interfering RNA for RAGE (siRAGE) into SW872 cells abolished the AGE- and HMGB1-induced up-regulation of IL-6 and CCL2. In addition, lipopolysaccharide (LPS), a ligand of RAGE, increased the expression of IL-6 and CCL2 and siRAGE attenuated the LPS-induced expression of IL-6 and CCL2. These results strongly suggest that the elevated AGE, HMGB1, and LPS in pregnant women up-regulate the expression of IL-6 and CCL2 via the RAGE system, leading to systemic inflammation such as PE/HDP. View Full-Text
Keywords: hypertensive disorders of pregnancy; RAGE; AGE; adipocyte; IL-6; CCL2; LPS hypertensive disorders of pregnancy; RAGE; AGE; adipocyte; IL-6; CCL2; LPS
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MDPI and ACS Style

Akasaka, J.; Naruse, K.; Sado, T.; Uchiyama, T.; Makino, M.; Yamauchi, A.; Ota, H.; Sakuramoto-Tsuchida, S.; Itaya-Hironaka, A.; Takasawa, S.; Kobayashi, H. Involvement of Receptor for Advanced Glycation Endproducts in Hypertensive Disorders of Pregnancy. Int. J. Mol. Sci. 2019, 20, 5462. https://doi.org/10.3390/ijms20215462

AMA Style

Akasaka J, Naruse K, Sado T, Uchiyama T, Makino M, Yamauchi A, Ota H, Sakuramoto-Tsuchida S, Itaya-Hironaka A, Takasawa S, Kobayashi H. Involvement of Receptor for Advanced Glycation Endproducts in Hypertensive Disorders of Pregnancy. International Journal of Molecular Sciences. 2019; 20(21):5462. https://doi.org/10.3390/ijms20215462

Chicago/Turabian Style

Akasaka, Juria, Katsuhiko Naruse, Toshiyuki Sado, Tomoko Uchiyama, Mai Makino, Akiyo Yamauchi, Hiroyo Ota, Sumiyo Sakuramoto-Tsuchida, Asako Itaya-Hironaka, Shin Takasawa, and Hiroshi Kobayashi. 2019. "Involvement of Receptor for Advanced Glycation Endproducts in Hypertensive Disorders of Pregnancy" International Journal of Molecular Sciences 20, no. 21: 5462. https://doi.org/10.3390/ijms20215462

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