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Open AccessArticle

Overexpression of BpCUC2 Influences Leaf Shape and Internode Development in Betula pendula

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(19), 4722;
Received: 5 July 2019 / Revised: 19 September 2019 / Accepted: 19 September 2019 / Published: 23 September 2019
(This article belongs to the Collection Genetics and Molecular Breeding in Plants)
The CUP-SHAPED COTYLEDON 2 (CUC2) gene, which is negatively regulated by microRNA164 (miR164), has been specifically linked to the regulation of leaf margin serration and the maintenance of phyllotaxy in model plants. However, few studies have investigated these effects in woody plants. In this study, we integrated genomic, transcriptomic, and physiology approaches to explore the function of BpCUC2 gene in Betula pendula growth and development. Our results showed that Betula pendula plants overexpressing BpCUC2, which is targeted by BpmiR164, exhibit shortened internodes and abnormal leaf shapes. Subsequent analysis indicated that the short internodes of BpCUC2 overexpressed transgenic lines and were due to decreased epidermal cell size. Moreover, transcriptome analysis, yeast one-hybrid assays, and ChIP-PCR suggested that BpCUC2 directly binds to the LTRECOREATCOR15 (CCGAC), CAREOSREP1 (CAACTC), and BIHD1OS (TGTCA) motifs of a series of IAA-related and cyclin-related genes to regulate expression. These results may be useful to our understanding of the functional role and genetic regulation of BpCUC2. View Full-Text
Keywords: BpCUC2; BpmiR164; leaf; internode; Betula pendula BpCUC2; BpmiR164; leaf; internode; Betula pendula
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MDPI and ACS Style

Liu, C.; Xu, H.; Han, R.; Wang, S.; Liu, G.; Chen, S.; Chen, J.; Bian, X.; Jiang, J. Overexpression of BpCUC2 Influences Leaf Shape and Internode Development in Betula pendula. Int. J. Mol. Sci. 2019, 20, 4722.

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