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Trefoil Factor 3 (TFF3) Is Involved in Cell Migration for Skeletal Repair
Open AccessArticle

Effect of Tff3 Deficiency and ER Stress in the Liver

1
Department of Molecular Medicine, Ruđer Bošković Institute, Bijenička 54, 10 000 Zagreb, Croatia
2
Institute of Functional and Clinical Anatomy, Faculty of Medicine, Friedrich-Alexander-University Erlangen-Nürnberg, 91051 Erlangen, Germany
3
Department of Histology and Embryology, Faculty of Medicine, University of Osijek, J. Huttlera 4, HR-31000 Osijek, Croatia
4
ERA Chaire Team, Proteomics Laboratory, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia
5
Department of Physiology and Immunology, Faculty of Medicine, University of Osijek, J. Huttlera 4, HR-31000 Osijek, Croatia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(18), 4389; https://doi.org/10.3390/ijms20184389
Received: 1 August 2019 / Revised: 26 August 2019 / Accepted: 29 August 2019 / Published: 6 September 2019
(This article belongs to the Special Issue TFF Peptides: Lectins in Mucosal Protection and More)
Endoplasmic reticulum (ER) stress, a cellular condition caused by the accumulation of unfolded proteins inside the ER, has been recognized as a major pathological mechanism in a variety of conditions, including cancer, metabolic and neurodegenerative diseases. Trefoil factor family (TFFs) peptides are present in different epithelial organs, blood supply, neural tissues, as well as in the liver, and their deficiency has been linked to the ER function. Complete ablation of Tff3 expression is observed in steatosis, and as the most prominent change in the early phase of diabetes in multigenic mouse models of diabesity. To elucidate the role of Tff3 deficiency on different pathologically relevant pathways, we have developed a new congenic mouse model Tff3−/−/C57BL6/N from a mixed background strain (C57BL6/N /SV129) by using a speed congenics approach. Acute ER stress was evoked by tunicamycin treatment, and mice were sacrificed after 24 h. Afterwards the effect of Tff3 deficiency was evaluated with regard to the expression of relevant oxidative and ER stress genes, relevant proinflammatory cytokines/chemokines, and the global protein content. The most dramatic change was noticed at the level of inflammation-related genes, while markers for unfolded protein response were not significantly affected. Ultrastructural analysis confirmed that the size of lipid vacuoles was affected as well. Since the liver acts as an important metabolic and immunological organ, the influence of Tff3 deficiency and physiological function possibly reflects on the whole organism. View Full-Text
Keywords: ER stress; trefoil peptide 3; liver; tunicamycin; proinflammatory cytokines ER stress; trefoil peptide 3; liver; tunicamycin; proinflammatory cytokines
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Šešelja, K.; Bazina, I.; Welss, J.; Schicht, M.; Paulsen, F.; Bijelić, N.; Rođak, E.; Horvatić, A.; Gelemanović, A.; Mihalj, M.; Baus Lončar, M. Effect of Tff3 Deficiency and ER Stress in the Liver. Int. J. Mol. Sci. 2019, 20, 4389.

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