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Open AccessArticle

Acute Cytotoxic Effects on Morphology and Mechanical Behavior in MCF-7 Induced by TiO2NPs Exposure

Department of Mathematics and Physics “E. De Giorgi”, University of Salento, Via Monteroni, 73100 Lecce, Italy
IIT—Italian Institute of Technology, via Morego 30, 16163 Genova, Italy
CNR Nanotec—Institute of Nanotechnology, 73100 Lecce, Italy
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(14), 3594;
Received: 20 May 2019 / Revised: 15 July 2019 / Accepted: 19 July 2019 / Published: 23 July 2019
(This article belongs to the Special Issue Biomechanics of Cell Membrane)
The side effects induced by nanoparticle exposure at a cellular level are one of the priority research topics due to the steady increase in the use of nanoparticles (NPs). Recently, the focus on cellular morphology and mechanical behavior is gaining relevance in order to fully understand the cytotoxic mechanisms. In this regard, we have evaluated the morphomechanical alteration in human breast adenocarcinoma cell line (MCF-7) exposed to TiO2NPs at two different concentrations (25 and 50 µg/mL) and two time points (24 and 48 h). By using confocal and atomic force microscopy, we demonstrated that TiO2NP exposure induces significant alterations in cellular membrane elasticity, due to actin proteins rearrangement in cytoskeleton, as calculated in correspondence to nuclear and cytoplasmic compartments. In this work, we have emphasized the alteration in mechanical properties of the cellular membrane, induced by nanoparticle exposure. View Full-Text
Keywords: biomechanics; scanning force microscopy; cytotoxicity biomechanics; scanning force microscopy; cytotoxicity
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Cascione, M.; De Matteis, V.; Mandriota, G.; Leporatti, S.; Rinaldi, R. Acute Cytotoxic Effects on Morphology and Mechanical Behavior in MCF-7 Induced by TiO2NPs Exposure. Int. J. Mol. Sci. 2019, 20, 3594.

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