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Open AccessArticle

N-Terminal (1→3)-β-d-Glucan Recognition Proteins from Insects Recognize the Difference in Ultra-Structures of (1→3)-β-d-Glucan

Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo 192-0392, Japan
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Int. J. Mol. Sci. 2019, 20(14), 3498; https://doi.org/10.3390/ijms20143498
Received: 23 May 2019 / Revised: 13 July 2019 / Accepted: 15 July 2019 / Published: 16 July 2019
(This article belongs to the Special Issue Effects of Beta Glucan)
Recognition of (1→3)-β-d-glucans (BGs) by invertebrate β-1,3-d-glucan recognition protein (BGRP) plays a significant role in the activation of Toll pathway and prophenoloxidase systems in insect host defense against fungal invasion. To examine the structure diversity of BGRPs for the recognition of physiochemically different BGs, the binding specificity of BGRPs cloned from four different insects to structure different BGs was characterized using ELISA. Recombinant BGRPs expressed as Fc-fusion proteins of human IgG1 bound to the solid phase of BGs. Based on the binding specificities, the BGRPs were categorized into two groups with different ultrastructures and binding characters; one group specifically binds BGs with triple-helical conformation, while the other group recognizes BGs with disordered conformations like single-helical or partially opened triple helix. The BGRPs from the silkworm and the Indian meal moth bound to the BGs with a triple-helical structure, whereas BGRPs from the red flour beetle and yellow mealworm beetle showed no binding to triple-helical BGs, but bound to alkaline-treated BGs that have a partially opened triple-helical conformation. This evidence suggests that the insect BGRPs can distinguish between different conformations of BGs and are equipped for determining the diversity of BG structures. View Full-Text
Keywords: β-d-glucan; glucan binding protein; host defense; innate immunity β-d-glucan; glucan binding protein; host defense; innate immunity
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MDPI and ACS Style

Adachi, Y.; Ishii, M.; Kanno, T.; Tetsui, J.; Ishibashi, K.-i.; Yamanaka, D.; Miura, N.; Ohno, N. N-Terminal (1→3)-β-d-Glucan Recognition Proteins from Insects Recognize the Difference in Ultra-Structures of (1→3)-β-d-Glucan. Int. J. Mol. Sci. 2019, 20, 3498. https://doi.org/10.3390/ijms20143498

AMA Style

Adachi Y, Ishii M, Kanno T, Tetsui J, Ishibashi K-i, Yamanaka D, Miura N, Ohno N. N-Terminal (1→3)-β-d-Glucan Recognition Proteins from Insects Recognize the Difference in Ultra-Structures of (1→3)-β-d-Glucan. International Journal of Molecular Sciences. 2019; 20(14):3498. https://doi.org/10.3390/ijms20143498

Chicago/Turabian Style

Adachi, Yoshiyuki; Ishii, Masaki; Kanno, Takashi; Tetsui, Junko; Ishibashi, Ken-ichi; Yamanaka, Daisuke; Miura, Noriko; Ohno, Naohito. 2019. "N-Terminal (1→3)-β-d-Glucan Recognition Proteins from Insects Recognize the Difference in Ultra-Structures of (1→3)-β-d-Glucan" Int. J. Mol. Sci. 20, no. 14: 3498. https://doi.org/10.3390/ijms20143498

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