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Resolved Hyperfine at L-band for High-Spin CoEDTA, A Model for Co Sites in Proteins

Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Int. J. Mol. Sci. 2019, 20(10), 2385; https://doi.org/10.3390/ijms20102385
Received: 16 April 2019 / Revised: 30 April 2019 / Accepted: 10 May 2019 / Published: 14 May 2019
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Abstract

Low-frequency electron paramagnetic resonance (EPR) spectra were obtained for the Co complex of ethylene diamine tetraacetic acid (CoEDTA). It was found that the cobalt hyperfine at geff-mid is better resolved at a low frequency, L-band (1.37 GHz), and not resolved at X-band (9.631 GHz), which is the conventional frequency used for most spectra for metal complexes. Resolved cobalt hyperfine lines lead to additional EPR parameters like A-mid for cobalt and a more-accurate determination of g-mid. Resolved hyperfine lines in the L-band, but not the S-band, spectra were obtained at a concentration of 1 mM. Knowing these additional EPR parameters provides a means to better determine the electron density in the ground state orbital for each cobalt complex, as well as to determine differences upon a change of ligation. If zinc sites can be replaced by cobalt, the cobalt spectra for these sites will enhance the characterization of the zinc sites. View Full-Text
Keywords: electron paramagnetic resonance; EPR; L-band; 1.37 GHz; high-spin CoEDTA; resolved Co hyperfine lines at geff-mid electron paramagnetic resonance; EPR; L-band; 1.37 GHz; high-spin CoEDTA; resolved Co hyperfine lines at geff-mid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Antholine, W.E. Resolved Hyperfine at L-band for High-Spin CoEDTA, A Model for Co Sites in Proteins. Int. J. Mol. Sci. 2019, 20, 2385.

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