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Int. J. Mol. Sci. 2018, 19(7), 2052; https://doi.org/10.3390/ijms19072052

Genome-Wide Characterization and Expression Analyses of Pleurotus ostreatus MYB Transcription Factors during Developmental Stages and under Heat Stress Based on de novo Sequenced Genome

1
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing 100081, China
*
Author to whom correspondence should be addressed.
Received: 22 June 2018 / Revised: 11 July 2018 / Accepted: 13 July 2018 / Published: 14 July 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Pleurotus ostreatus is a commercially grown mushroom species in China. However, studies on the mechanisms of the fruiting body development and stress response of P. ostreatus are still at a primary stage. In this study, we report the entire genome sequence of P. ostreatus CCMSSC03989. Then, we performed comprehensive genome-wide characterization and expression analysis of the MYB transcription factor family during a series of developmental stages and under the condition of heat stress. A 34.76 Mb genome was obtained through next-generation sequencing (NGS) and Bionano optical mapping approaches. The genome has a scaffold N50 of 1.1 Mb and contains 10.11% repeats, and 10,936 gene models were predicted. A total of 20 MYB genes (PoMYB) were identified across the genome, and the full-length open reading frames were isolated. The PoMYBs were classified into 1 repeat (1R), 2R, and 3R-MYB groups according to their MYB domain repeat numbers, and 3R-MYBs possessed relatively more introns than 1R and 2R-MYBs. Based on phylogenetic analysis, the PoMYBs were divided into four groups and showed close relationships with the MYB genes of plants and fungi. RNA-sequencing (RNA-Seq) and quantitative PCR (qPCR) analyses revealed that PoMYB expression showed stage-specific patterns in reproductive stages and could be induced by heat stress. The P. ostreatus draft genome will promote genome-wide analysis, and our study of PoMYBs will promote further functional analysis of MYB genes in mushrooms. View Full-Text
Keywords: Pleurotus ostreatus; de novo; NGS; Bionano; MYB; development; heat stress Pleurotus ostreatus; de novo; NGS; Bionano; MYB; development; heat stress
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Wang, L.; Gao, W.; Wu, X.; Zhao, M.; Qu, J.; Huang, C.; Zhang, J. Genome-Wide Characterization and Expression Analyses of Pleurotus ostreatus MYB Transcription Factors during Developmental Stages and under Heat Stress Based on de novo Sequenced Genome. Int. J. Mol. Sci. 2018, 19, 2052.

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