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Int. J. Mol. Sci. 2018, 19(6), 1751; https://doi.org/10.3390/ijms19061751

Whole-Transcriptome Sequence Analysis of Verbena bonariensis in Response to Drought Stress

1
Department of Ornamental Horticulture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, Sichuan, China
2
Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center, The Chinese Academy of Science, Shanghai Chenshan Botanical Garden, 3888 Huagong Road, Songjiang District, Shanghai 201602, China
*
Authors to whom correspondence should be addressed.
Received: 20 April 2018 / Revised: 5 June 2018 / Accepted: 8 June 2018 / Published: 13 June 2018
(This article belongs to the Special Issue Plant Genomics)
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Abstract

Drought is an important abiotic factor that threatens the growth and development of plants. Verbena bonariensis is a widely used landscape plant with a very high ornamental value. We found that Verbena has drought tolerance in production practice, so in order to delve into its mechanism of drought resistance and screen out its drought-resistance genes, we used the RNA-Seq platform to perform a de novo transcriptome assembly to analyze Verbena transcription response to drought stress. By high-throughput sequencing with Illumina Hiseq Xten, a total of 44.59 Gb clean data was obtained from T01 (control group) and T02 (drought experiment group). After assembly, 111,313 unigenes were obtained, and 53,757 of them were annotated by compared databases. In this study, 4829 differentially expressed genes were obtained, of which 4165 were annotated. We performed GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analyses, and explored a lot of differently expressed genes related to plant energy production, hormone synthesis, cell signal transduction, and metabolism to understand the stress response of Verbena in drought stress. In addition, we also found that a series of TFs related to drought-resistance of Verbena and provide excellent genetic resources for improving the drought tolerance of crops. View Full-Text
Keywords: Verbena bonariensis; drought stress; transcriptome sequencing; differentially expressed genes Verbena bonariensis; drought stress; transcriptome sequencing; differentially expressed genes
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Wang, B.; Lv, X.-Q.; He, L.; Zhao, Q.; Xu, M.-S.; Zhang, L.; Jia, Y.; Zhang, F.; Liu, F.-L.; Liu, Q.-L. Whole-Transcriptome Sequence Analysis of Verbena bonariensis in Response to Drought Stress. Int. J. Mol. Sci. 2018, 19, 1751.

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