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Int. J. Mol. Sci. 2018, 19(6), 1700; https://doi.org/10.3390/ijms19061700

Keratinocyte Motility Is Affected by UVA Radiation—A Comparison between Normal and Dysplastic Cells

1
Victor Babeș National Institute of Pathology, 99-101, Splaiul Independentei, 050096 Bucharest, Romania
2
Department of Morphological Sciences, University of Medicine and Pharmacy Carol Davila, 8, Blvd. Eroilor Sanitari, 050474 Bucharest, Romania
3
Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, 91-95, Splaiul Independentei, 050095 Bucharest, Romania
4
SC Biotehnos SA, 3-5, Gorunului Street, 075100 Otopeni, Romania
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 3 May 2018 / Revised: 1 June 2018 / Accepted: 5 June 2018 / Published: 7 June 2018
(This article belongs to the Section Biochemistry)
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Abstract

UVA radiation induces multiple and complex changes in the skin, affecting epidermal cell behavior. This study reports the effects of UVA exposure on normal (HaCaT) and dysplastic (DOK) keratinocytes. The adherence, spreading and proliferation were investigated by time-lapse measurement of cell layer impedance on different matrix proteins. Prior to UVA exposure, the time required for adherence and spreading did not differ significantly for HaCaT and DOK cells, while spreading areas were larger for HaCaT cells. Under UVA exposure, HaCaT and DOK cells behavior differed in terms of movement and proliferation. The cells’ ability to cover the denuded surface and individual cell trajectories were recorded by time-lapse videomicroscopy, during wound healing experiments. Dysplastic keratinocytes showed more sensitivity to UVA, exhibiting transient deficiencies in directionality of movement and a delay in re-coating the denuded area. The actin cytoskeleton displayed a cortical organization immediately after irradiation, in both cell lines, similar to mock-irradiated cells. Post-irradiation, DOK cells displayed a better organization of stress fibers, persistent filopodia, and new, stronger focal contacts. In conclusion, after UVA exposure HaCaT and DOK cells showed a different behavior in terms of adherence, spreading, motility, proliferation, and actin cytoskeleton dynamics, with the dyplastic keratinocytes being more sensitive. View Full-Text
Keywords: cell motility; keratinocyte; dysplastic cell; wound healing; actin cytoskeleton; focal contacts cell motility; keratinocyte; dysplastic cell; wound healing; actin cytoskeleton; focal contacts
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Niculiţe, C.M.; Nechifor, M.T.; Urs, A.O.; Olariu, L.; Ceafalan, L.C.; Leabu, M. Keratinocyte Motility Is Affected by UVA Radiation—A Comparison between Normal and Dysplastic Cells. Int. J. Mol. Sci. 2018, 19, 1700.

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