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Chemical Exposure-Induced Changes in the Expression of Neurotrophins and Their Receptors in the Main Olfactory System of Mice Lacking TRPM5-Expressing Microvillous Cells

Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD 21250, USA
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Int. J. Mol. Sci. 2018, 19(10), 2939; https://doi.org/10.3390/ijms19102939
Received: 12 July 2018 / Revised: 25 August 2018 / Accepted: 24 September 2018 / Published: 27 September 2018
(This article belongs to the Section Biochemistry)
Functional maintenance of the mammalian main olfactory epithelium (MOE) is challenging because of its direct exposure to a wide spectrum of environmental chemicals. We previously reported that transient receptor potential channel M5-expressing microvillous cells (TRPM5-MCs) in the MOE play an important role in olfactory maintenance. To investigate the underpinning mechanisms, we exposed transcription factor Skn-1a knockout (Skn-1a−/−) mice lacking TRPM5-MCs, and TRPM5-GFP mice to either vehicle (water) or a mixture of odorous chemicals and chitin for two weeks and analyzed the expression of olfactory signaling proteins using immunolabeling and neurotrophin (NT) and NT receptor (NTR) gene transcripts using real-time quantitative PCR. The chemical exposure did not significantly attenuate the immunolabeling of olfactory signaling proteins. Vehicle-exposed Skn-1a−/− and TRPM5-GFP mice expressed similar levels of NT and NTR gene transcripts in the MOE and olfactory bulb. Chemical exposure significantly increased MOE expression of p75NTR in Skn-1a−/− mice, while p75NTR expression was reduced in TRPM5-GFP mice, as compared to vehicle-exposed mice. Additionally, our RNA in situ hybridization analysis and immunolabeling confirmed MOE expression of most NTs and NTRs. Together, these results indicate that TRPM5-MCs and chemical exposure influence expression of some NTs and NTRs in the MOE and olfactory bulb (OB). View Full-Text
Keywords: olfactory epithelial maintenance; real-time qPCR; olfactory sensory neurons; microvillous cells; Skn-1a/Pou2f3 olfactory epithelial maintenance; real-time qPCR; olfactory sensory neurons; microvillous cells; Skn-1a/Pou2f3
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AlMatrouk, A.; Lemons, K.; Ogura, T.; Luo, W.; Wilson, C.; Lin, W. Chemical Exposure-Induced Changes in the Expression of Neurotrophins and Their Receptors in the Main Olfactory System of Mice Lacking TRPM5-Expressing Microvillous Cells. Int. J. Mol. Sci. 2018, 19, 2939.

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