Next Article in Journal
Effective Sequential Combined Chemotherapy with Trifluridine/Tipiracil and Regorafenib in Human Colorectal Cancer Cells
Previous Article in Journal
An In Vitro Model of Angiogenesis during Wound Healing Provides Insights into the Complex Role of Cells and Factors in the Inflammatory and Proliferation Phase
Open AccessArticle

Gummy Stem Blight Resistance in Melon: Inheritance Pattern and Development of Molecular Markers

Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Korea
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(10), 2914;
Received: 30 August 2018 / Revised: 20 September 2018 / Accepted: 22 September 2018 / Published: 25 September 2018
(This article belongs to the Section Molecular Plant Sciences)
Gummy stem blight (GSB) causes enormous losses to melon (Cucumis melo L.) production worldwide. We aimed to develop useful molecular markers linked to GSB resistance. In this study, 168 F2 plants were obtained from the F1 population of a cross between the GSB-susceptible ‘Cornell ZPPM 339’ and the GSB-resistant ‘PI482399’ lines. A 3:1 ratio of susceptible and resistant genotypes was observed in the F2 population, indicating control by a single recessive gene. Nucleotide-binding site leucine-rich repeat (NBS-LRR) genes confer resistance against insects and diseases in cucurbits including melon. We cloned and sequenced the TIR-NBS-LRR-type resistance gene MELO3C022157, located on melon chromosome 9, from resistant and susceptible lines. Sequence analysis revealed deletions in the first intron, a 2-bp frameshift deletion from the second exon and a 7-bp insertion in the 4th exon of the resistant line. We developed two insertion/deletion (InDel) markers, GSB9-kh-1 and GSB9-kh-2, which were found in the first intron of MELO3C022157 linked to GSB resistance. We validated these markers with the F2 population and inbred lines. These InDels may be used to facilitate marker-assisted selection of GSB resistance in melon. However, functional analysis of overexpressing and/or knock-down mutants is needed to confirm the frameshift mutation. View Full-Text
Keywords: inheritance; GSB; NBS-LRR; InDel; phenotype; genotype inheritance; GSB; NBS-LRR; InDel; phenotype; genotype
Show Figures

Figure 1

MDPI and ACS Style

Hassan, M.Z.; Rahim, M.A.; Natarajan, S.; Robin, A.H.K.; Kim, H.-T.; Park, J.-I.; Nou, I.-S. Gummy Stem Blight Resistance in Melon: Inheritance Pattern and Development of Molecular Markers. Int. J. Mol. Sci. 2018, 19, 2914.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop