Next Article in Journal
Ethnobotanic, Ethnopharmacologic Aspects and New Phytochemical Insights into Moroccan Argan Fruits
Next Article in Special Issue
Genome Analysis and Genetic Stability of the Cryptophlebia leucotreta Granulovirus (CrleGV-SA) after 15 Years of Commercial Use as a Biopesticide
Previous Article in Journal
Modulation of the Senescence-Associated Inflammatory Phenotype in Human Fibroblasts by Olive Phenols
Previous Article in Special Issue
Unexpected High Intragenomic Variation in Two of Three Major Pest Thrips Species Does Not Affect Ribosomal Internal Transcribed Spacer 2 (ITS2) Utility for Thrips Identification
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(11), 2279; https://doi.org/10.3390/ijms18112279

PeaTAR1B: Characterization of a Second Type 1 Tyramine Receptor of the American Cockroach, Periplaneta americana

1
Faculty of Biology, Phillips University of Marburg, 35037 Marburg, Germany
2
Institute of Complex Systems—Cellular Biophysics (ICS-4), Forschungszentrum Jülich, 52428 Jülich, Germany
*
Author to whom correspondence should be addressed.
Received: 11 September 2017 / Revised: 22 October 2017 / Accepted: 26 October 2017 / Published: 30 October 2017
(This article belongs to the Special Issue Molecular Entomology of Insects of Economic Importance)
Full-Text   |   PDF [1997 KB, uploaded 31 October 2017]   |  

Abstract

The catecholamines norepinephrine and epinephrine regulate important physiological functions in vertebrates. In insects; these neuroactive substances are functionally replaced by the phenolamines octopamine and tyramine. Phenolamines activate specific guanine nucleotide-binding (G) protein-coupled receptors (GPCRs). Type 1 tyramine receptors are better activated by tyramine than by octopamine. In contrast; type 2 tyramine receptors are almost exclusively activated by tyramine. Functionally; activation of type 1 tyramine receptors leads to a decrease in the intracellular concentration of cAMP ([cAMP]i) whereas type 2 tyramine receptors can mediate Ca2+ signals or both Ca2+ signals and effects on [cAMP]i. Here; we report that the American cockroach (Periplaneta americana) expresses a second type 1 tyramine receptor (PeaTAR1B) in addition to PeaTAR1A (previously called PeaTYR1). When heterologously expressed in flpTM cells; activation of PeaTAR1B by tyramine leads to a concentration-dependent decrease in [cAMP]i. Its activity can be blocked by a series of established antagonists. The functional characterization of two type 1 tyramine receptors from P. americana; PeaTAR1A and PeaTAR1B; which respond to tyramine by changing cAMP levels; is a major step towards understanding the actions of tyramine in cockroach physiology and behavior; particularly in comparison to the effects of octopamine. View Full-Text
Keywords: cAMP; biogenic amines; Ca2+ fluorimetry; cell-based assay; cellular signaling; G protein-coupled receptor; insect; octopamine; second messenger cAMP; biogenic amines; Ca2+ fluorimetry; cell-based assay; cellular signaling; G protein-coupled receptor; insect; octopamine; second messenger
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Blenau, W.; Balfanz, S.; Baumann, A. PeaTAR1B: Characterization of a Second Type 1 Tyramine Receptor of the American Cockroach, Periplaneta americana. Int. J. Mol. Sci. 2017, 18, 2279.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top