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Open AccessArticle

Genetic Diversity and Population Structure of Broomcorn Millet (Panicum miliaceum L.) Cultivars and Landraces in China Based on Microsatellite Markers

1
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
School of Life Science, Jilin University, Changchun 130012, China
3
Institute of Crop Science, Gansu Academy of Agricultural Sciences, Lanzhou 030000, China
4
Faculty of Life Science, Jilin Agricultural University, Changchun 130118, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Int. J. Mol. Sci. 2016, 17(3), 370; https://doi.org/10.3390/ijms17030370
Received: 30 December 2015 / Revised: 2 March 2016 / Accepted: 8 March 2016 / Published: 14 March 2016
(This article belongs to the Special Issue Plant Molecular Biology)
Broomcorn millet (Panicum miliaceum L.), one of the first domesticated crops, has been grown in Northern China for at least 10,000 years. The species is presently a minor crop, and evaluation of its genetic diversity has been very limited. In this study, we analyzed the genetic diversity of 88 accessions of broomcorn millet collected from various provinces of China. Amplification with 67 simple sequence repeat (SSR) primers revealed moderate levels of diversity in the investigated accessions. A total of 179 alleles were detected, with an average of 2.7 alleles per locus. Polymorphism information content and expected heterozygosity ranged from 0.043 to 0.729 (mean = 0.376) and 0.045 to 0.771 (mean = 0.445), respectively. Cluster analysis based on the unweighted pair group method of mathematical averages separated the 88 accessions into four groups at a genetic similarity level of 0.633. A genetic structure assay indicated a close correlation between geographical regions and genetic diversity. The uncovered information will be valuable for defining gene pools and developing breeding programs for broomcorn millet. Furthermore, the millet-specific SSR markers developed in this study should serve as useful tools for assessment of genetic diversity and elucidation of population structure in broomcorn millet. View Full-Text
Keywords: genetic diversity; population structure; SSR markers; Panicum miliaceum L. varieties genetic diversity; population structure; SSR markers; Panicum miliaceum L. varieties
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MDPI and ACS Style

Liu, M.; Xu, Y.; He, J.; Zhang, S.; Wang, Y.; Lu, P. Genetic Diversity and Population Structure of Broomcorn Millet (Panicum miliaceum L.) Cultivars and Landraces in China Based on Microsatellite Markers. Int. J. Mol. Sci. 2016, 17, 370.

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