Microarray Study of Pathway Analysis Expression Profile Associated with MicroRNA-29a with Regard to Murine Cholestatic Liver Injuries
Abstract
:1. Introduction
2. Results
2.1. Gain of miR-29a Signaling Considerably Mitigates Liver Fibrosis in Cholestatic Livers
2.2. Analyses of Microarray Data
2.3. Overexpression of miR-29a Considerably Hinders TGF-β Signaling Pathways in Cholestatic Livers
2.4. Overexpression of miR-29a Considerably Increased Wnt/β-Catenin in Cholestatic Livers
2.5. Overexpression of miR-29a Considerably Increased Dickkopf-Related Protein 1 (Dkk1) Expression in Cholestatic Livers
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Greation of miR-29a Transgenic Mice
4.3. Animal Model and Experimental Protocol
4.4. Histological Analysis
4.5. Microarray Samples
4.6. mRNA Expression Detected by Real-Time Quantitative RT-PCR
4.7. Immunoblotting
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Pathway Name | p Value | # of Genes in the Pathway | Pathway ID |
---|---|---|---|
Metabolic pathways | 0.000001 | 145 | kegg_pathway_235 |
Peroxisome | 0.000229 | 19 | kegg_pathway_157 |
Cell cycle | 0.000548 | 24 | kegg_pathway_53 |
Retinol metabolism | 0.000743 | 16 | kegg_pathway_57 |
Steroid hormone biosynthesis | 0.000904 | 14 | kegg_pathway_294 |
Protein digestion and absorption | 0.001179 | 16 | kegg_pathway_206 |
Arachidonic acid metabolism | 0.002021 | 15 | kegg_pathway_38 |
Biosynthesis of antibiotics | 0.002177 | 31 | kegg_pathway_274 |
Lysine degradation | 0.003177 | 13 | kegg_pathway_203 |
Fatty acid degradation | 0.004790 | 11 | kegg_pathway_125 |
TGF-β signaling pathway | 0.009331 | 15 | kegg_pathway_93 |
Linoleic acid metabolism | 0.010929 | 8 | kegg_pathway_263 |
Amoebiasis | 0.011037 | 18 | kegg_pathway_111 |
Tryptophan metabolism | 0.012916 | 9 | kegg_pathway_88 |
Progesterone-mediated oocyte maturation | 0.014029 | 16 | kegg_pathway_200 |
ECM-receptor interaction | 0.015281 | 14 | kegg_pathway_147 |
Chemical carcinogenesis | 0.018625 | 12 | kegg_pathway_229 |
Butanoate metabolism | 0.025619 | 6 | kegg_pathway_248 |
Bile secretion | 0.027883 | 12 | kegg_pathway_291 |
Oocyte meiosis | 0.029539 | 17 | kegg_pathway_230 |
Small cell lung cancer | 0.030371 | 14 | kegg_pathway_190 |
PPAR signaling pathway | 0.033269 | 13 | kegg_pathway_78 |
Regulation of lipolysis in adipocytes | 0.037877 | 10 | kegg_pathway_255 |
Histidine metabolism | 0.038958 | 5 | kegg_pathway_165 |
Drug metabolism—other enzymes | 0.039071 | 8 | kegg_pathway_36 |
Fatty acid metabolism | 0.046776 | 9 | kegg_pathway_27 |
Fatty acid biosynthesis | 0.048322 | 4 | kegg_pathway_89 |
Mineral absorption | 0.049102 | 8 | kegg_pathway_236 |
Pathway Name | p Value | # of Genes in the Pathway | Pathway ID |
---|---|---|---|
Steroid hormone biosynthesis | 0.000021 | 8 | kegg_pathway_294 |
Leukocyte transendothelial migration | 0.000029 | 11 | kegg_pathway_276 |
Natural killer cell mediated cytotoxicity | 0.000347 | 10 | kegg_pathway_253 |
Chemical carcinogenesis | 0.000356 | 7 | kegg_pathway_229 |
Fc epsilon RI signaling pathway | 0.000914 | 7 | kegg_pathway_100 |
Platelet activation | 0.001147 | 9 | kegg_pathway_23 |
B cell receptor signaling pathway | 0.001389 | 7 | kegg_pathway_65 |
Osteoclast differentiation | 0.002132 | 9 | kegg_pathway_135 |
Arrhythmogenic right ventricular cardiomyopathy (ARVC) | 0.002642 | 6 | kegg_pathway_1 |
Retinol metabolism | 0.002727 | 6 | kegg_pathway_57 |
Bile secretion | 0.002771 | 6 | kegg_pathway_291 |
Phagosome | 0.003856 | 10 | kegg_pathway_194 |
Hypertrophic cardiomyopathy (HCM) | 0.005919 | 6 | kegg_pathway_279 |
Energy metabolism | 0.011458 | 8 | kegg_pathway_16 |
Pentose and glucuronate interconversions | 0.021195 | 3 | kegg_pathway_285 |
Metabolic pathways | 0.021733 | 31 | kegg_pathway_235 |
Focal adhesion | 0.022124 | 9 | kegg_pathway_187 |
Inositol phosphate metabolism | 0.022784 | 4 | kegg_pathway_49 |
Viral carcinogenesis | 0.024087 | 9 | kegg_pathway_216 |
Axon guidance | 0.025580 | 7 | kegg_pathway_264 |
Antigen processing and presentation | 0.025778 | 5 | kegg_pathway_269 |
Viral myocarditis | 0.026037 | 5 | kegg_pathway_47 |
Linoleic acid metabolism | 0.027978 | 3 | kegg_pathway_263 |
Dilated cardiomyopathy | 0.029280 | 5 | kegg_pathway_277 |
Autoimmune thyroid disease | 0.039891 | 4 | kegg_pathway_64 |
Biosynthesis of antibiotics | 0.040668 | 8 | kegg_pathway_274 |
Fc gamma R-mediated phagocytosis | 0.041882 | 5 | kegg_pathway_120 |
2-Oxocarboxylic acid metabolism | 0.046164 | 2 | kegg_pathway_35 |
HTLV-I infection | 0.047823 | 10 | kegg_pathway_189 |
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Li, S.-C.; Wang, F.-S.; Yang, Y.-L.; Tiao, M.-M.; Chuang, J.-H.; Huang, Y.-H. Microarray Study of Pathway Analysis Expression Profile Associated with MicroRNA-29a with Regard to Murine Cholestatic Liver Injuries. Int. J. Mol. Sci. 2016, 17, 324. https://doi.org/10.3390/ijms17030324
Li S-C, Wang F-S, Yang Y-L, Tiao M-M, Chuang J-H, Huang Y-H. Microarray Study of Pathway Analysis Expression Profile Associated with MicroRNA-29a with Regard to Murine Cholestatic Liver Injuries. International Journal of Molecular Sciences. 2016; 17(3):324. https://doi.org/10.3390/ijms17030324
Chicago/Turabian StyleLi, Sung-Chou, Feng-Sheng Wang, Ya-Ling Yang, Mao-Meng Tiao, Jiin-Haur Chuang, and Ying-Hsien Huang. 2016. "Microarray Study of Pathway Analysis Expression Profile Associated with MicroRNA-29a with Regard to Murine Cholestatic Liver Injuries" International Journal of Molecular Sciences 17, no. 3: 324. https://doi.org/10.3390/ijms17030324