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Int. J. Mol. Sci. 2016, 17(12), 2117;

Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers

Department of Pathology, Erasme Hospital, Université Libre de Bruxelles, 1070 Brussels, Belgium
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 27 October 2016 / Revised: 1 December 2016 / Accepted: 9 December 2016 / Published: 16 December 2016
(This article belongs to the Special Issue Next-Generation Sequencing for Clinical Application)
Full-Text   |   PDF [212 KB, uploaded 16 December 2016]


Promising targeted therapy and personalized medicine are making molecular profiling of tumours a priority. For colorectal cancer (CRC) patients, international guidelines made RAS (KRAS and NRAS) status a prerequisite for the use of anti-epidermal growth factor receptor agents (anti-EGFR). Daily, new data emerge on the theranostic and prognostic role of molecular biomarkers, which is a strong incentive for a validated, sensitive and broadly available molecular screening test in order to implement and improve multi-modal therapy strategy and clinical trials. Next generation sequencing (NGS) has begun to supplant other technologies for genomic profiling. Targeted NGS is a method that allows parallel sequencing of thousands of short DNA sequences in a single test offering a cost-effective approach for detecting multiple genetic alterations with a minimum amount of DNA. In the present review, we collected data concerning the clinical application of NGS technology in the setting of colorectal cancer. View Full-Text
Keywords: targeted next generation sequencing; colorectal cancer; clinical application targeted next generation sequencing; colorectal cancer; clinical application
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Fontanges, Q.; De Mendonca, R.; Salmon, I.; Le Mercier, M.; D’Haene, N. Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers. Int. J. Mol. Sci. 2016, 17, 2117.

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