Next Article in Journal
Potential of LC Coupled to Fluorescence Detection in Food Metabolomics: Determination of Phenolic Compounds in Virgin Olive Oil
Next Article in Special Issue
The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B
Previous Article in Journal
miR33a/miR33b* and miR122 as Possible Contributors to Hepatic Lipid Metabolism in Obese Women with Nonalcoholic Fatty Liver Disease
Previous Article in Special Issue
New Natural Pigment Fraction Isolated from Saw Palmetto: Potential for Adjuvant Therapy of Hepatocellular Carcinoma
Open AccessArticle

Direct LAMP Assay without Prior DNA Purification for Sex Determination of Papaya

by Chi-Chu Tsai 1,2,†, Huei-Chuan Shih 3,†, Ya-Zhu Ko 4,†, Ren-Huang Wang 1,†, Shu-Ju Li 1 and Yu-Chung Chiang 4,5,*
Kaohsiung District Agricultural Research and Extension Station, Pingtung 908, Taiwan
Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
Department of Nursing, Meiho University, Pingtung 912, Taiwan
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan
Department of Biomedical Science and Environment Biology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Author to whom correspondence should be addressed.
Academic Editor: Chang Won Choi
Int. J. Mol. Sci. 2016, 17(10), 1630;
Received: 18 August 2016 / Revised: 15 September 2016 / Accepted: 20 September 2016 / Published: 24 September 2016
(This article belongs to the Special Issue Plant-Derived Pharmaceuticals by Molecular Farming 2016)
Papaya (Carica papaya L.) is an economically important tropical fruit tree with hermaphrodite, male and female sex types. Hermaphroditic plants are the major type used for papaya production because their fruits have more commercial advantages than those of female plants. Sex determination of the seedlings, or during the early growth stages, is very important for the papaya seedling industry. Thus far, the only method for determining the sex type of a papaya at the seedling stage has been DNA analysis. In this study, a molecular technique—based on DNA analysis—was developed for detecting male-hermaphrodite-specific markers to examine the papaya’s sex type. This method is based on the loop-mediated isothermal amplification (LAMP) and does not require prior DNA purification. The results show that the method is an easy, efficient, and inexpensive way to determine a papaya’s sex. This is the first report on the LAMP assay, using intact plant materials-without DNA purification-as samples for the analysis of sex determination of papaya. We found that using high-efficiency DNA polymerase was essential for successful DNA amplification, using trace intact plant material as a template DNA source. View Full-Text
Keywords: papaya; sex determination; loop-mediated isothermal amplification (LAMP); without DNA purification papaya; sex determination; loop-mediated isothermal amplification (LAMP); without DNA purification
Show Figures

Graphical abstract

MDPI and ACS Style

Tsai, C.-C.; Shih, H.-C.; Ko, Y.-Z.; Wang, R.-H.; Li, S.-J.; Chiang, Y.-C. Direct LAMP Assay without Prior DNA Purification for Sex Determination of Papaya. Int. J. Mol. Sci. 2016, 17, 1630.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop