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Int. J. Mol. Sci. 2015, 16(5), 9504-9519;

An Exploratory Pilot Study of Genetic Marker for IgE-Mediated Allergic Diseases with Expressions of FcεR1α and Cε

Center for Translational Medicine, Department of Medical Research, Taichung Veterans General Hospital, Taichung 40705, Taiwan
Department of Bio-Industry Technology, Da Yeh University, Changhua 51591, Taiwan
Department of Medical Technology, Jen Ten College of Medicine, Nursing and Management, Miaoli 35664, Taiwan
Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 40705, Taiwan
College of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan
Institute of Clinical Medicine, School of Medicine, National Yang Ming University, Taipei 11221, Taiwan
Author to whom correspondence should be addressed.
Academic Editor: Camile S. Farah
Received: 11 December 2014 / Revised: 10 April 2015 / Accepted: 20 April 2015 / Published: 27 April 2015
(This article belongs to the Special Issue Emerging Classes of Biomarkers for Molecular Diagnostics)
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The high affinity immunoglobulin E (IgE) receptor-FcεR1 is mainly expressed on the surface of effector cells. Cross-linking of IgE Abs bound to FcεR1 by multi-valent antigens can induce the activation of these cells and the secretion of inflammatory mediators. Since FcεR1 plays a central role in the induction and maintenance of allergic responses, this study aimed to investigate the association of FcεR1 with the allergic phenotype of Cε expression and cytokine and histamine release from peripheral leukocytes. Peripheral leukocytes from 67 allergic and 50 non-allergic subjects were used for genotyping analysis. Peripheral mononuclear cells (PBMCs) were used for Cε expression and ELISpot analysis, while polymorphonuclear cells (PMNs) were used for histamine release. The association between genotype polymorphism of the FcεR1α promoter region (rs2427827 and rs2251746) and allergic features of Cε expression and histamine were analyzed, and their effects on leukocytes function were compared with wild type. The genotype polymorphisms of FcεR1α promoter region with CT and TT in rs2427827 and TC in rs2251746 were significantly higher in allergic patients than in non-allergic controls. Patients with single nucleotide polymorphism (SNP) of FcεR1α promoter region had high levels of total IgE, mite-specific Der p 2 (Group 2 allergen of Dermatophagoides pteronyssinus)-specific IgE and IgE secretion B cells. The mRNA expression of FcεR1α was significantly increased after Der p2 stimulation in PBMCs with SNPs of the FcεR1α promoter region. Despite the increased Cε mRNA expression in PBMCs and histamine release from PMNs and the up-regulated mRNA expression of interleukin (IL)-6 and IL-8 secretions after Der p2 stimulation, there was no statistically significant difference between SNPs of the FcεR1α promoter region and the wild type. SNPs of FcεR1α promoter region were associated with IgE expression, IgE producing B cells, and increased Der p2-induced FcεR1α mRNA expression. These SNPs may be used as a disease marker for IgE-mediated allergic inflammation caused by Dermatophagoides pteronyssinus. View Full-Text
Keywords: FcεR1α promoter region; FcεR1α mRNA expression; single nucleotide polymorphism (SNP); genetic markers; allergic diseases FcεR1α promoter region; FcεR1α mRNA expression; single nucleotide polymorphism (SNP); genetic markers; allergic diseases

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Liao, E.-C.; Chang, C.-Y.; Hsieh, C.-W.; Yu, S.-J.; Yin, S.-C.; Tsai, J.-J. An Exploratory Pilot Study of Genetic Marker for IgE-Mediated Allergic Diseases with Expressions of FcεR1α and Cε. Int. J. Mol. Sci. 2015, 16, 9504-9519.

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