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Single Cell Confocal Raman Spectroscopy of Human Osteoarthritic Chondrocytes: A Preliminary Study

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Department of Physics, Norwegian University of Science and Technology (NTNU), N-7491 Trondheim, Norway
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Physical Optics and Electronics Group, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA
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Orthopaedic Department, Levanger Hospital, Kirkegata 2, N-7600 Levanger, Norway
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Nofima, Postbox 210, N-1431 Ås, Norway
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Department of Orthopaedic Surgery, Trondheim University Hospital, N-7491 Trondheim, Norway
*
Author to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Int. J. Mol. Sci. 2015, 16(5), 9341-9353; https://doi.org/10.3390/ijms16059341
Received: 19 March 2015 / Revised: 18 April 2015 / Accepted: 20 April 2015 / Published: 24 April 2015
(This article belongs to the Section Biochemistry)
A great deal of effort has been focused on exploring the underlying molecular mechanism of osteoarthritis (OA) especially at the cellular level. We report a confocal Raman spectroscopic investigation on human osteoarthritic chondrocytes. The objective of this investigation is to identify molecular features and the stage of OA based on the spectral signatures corresponding to bio-molecular changes at the cellular level in chondrocytes. In this study, we isolated chondrocytes from human osteoarthritic cartilage and acquired Raman spectra from single cells. Major spectral differences between the cells obtained from different International Cartilage Repair Society (ICRS) grades of osteoarthritic cartilage were identified. During progression of OA, a decrease in protein content and an increase in cell death were observed from the vibrational spectra. Principal component analysis and subsequent cross-validation was able to associate osteoarthritic chondrocytes to ICRS Grade I, II and III with specificity 100.0%, 98.1%, and 90.7% respectively, while, sensitivity was 98.6%, 82.8%, and 97.5% respectively. The overall predictive efficiency was 92.2%. Our pilot study encourages further use of Raman spectroscopy as a noninvasive and label free technique for revealing molecular features associated with osteoarthritic chondrocytes. View Full-Text
Keywords: Raman spectroscopy; osteoarthritis; chondrocytes; biomedical optical diagnosis Raman spectroscopy; osteoarthritis; chondrocytes; biomedical optical diagnosis
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Kumar, R.; Singh, G.P.; Grønhaug, K.M.; Afseth, N.K.; De Lange Davies, C.; Drogset, J.O.; Lilledahl, M.B. Single Cell Confocal Raman Spectroscopy of Human Osteoarthritic Chondrocytes: A Preliminary Study. Int. J. Mol. Sci. 2015, 16, 9341-9353.

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