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Open AccessArticle

Dual Targeting Biomimetic Liposomes for Paclitaxel/DNA Combination Cancer Treatment

by Guo-Xia Liu 1, Gui-Qing Fang 1 and Wei Xu 2,*
Department of Clinical Lab, Jinan Stomatological Hospital, Jinan 250001, China
Department of Pharmacy, Shandong Provincial Qian Foshan Hospital, Jinan 250014, China
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(9), 15287-15303;
Received: 7 May 2014 / Revised: 15 August 2014 / Accepted: 21 August 2014 / Published: 29 August 2014
(This article belongs to the Special Issue Biomimetic and Functional Materials)
Combinations of chemotherapeutic drugs with nucleic acid has shown great promise in cancer therapy. In the present study, paclitaxel (PTX) and DNA were co-loaded in the hyaluronic acid (HA) and folate (FA)-modified liposomes (HA/FA/PPD), to obtain the dual targeting biomimetic nanovector. The prepared HA/FA/PPD exhibited nanosized structure and narrow size distributions (247.4 ± 4.2 nm) with appropriate negative charge of −25.40 ± 2.7 mV. HA/FA/PD (PTX free HA/FA/PPD) showed almost no toxicity on murine malignant melanoma cell line (B16) and human hepatocellular carcinoma cell line (HepG2) (higher than 80% cell viability), demonstrating the safety of the blank nanovector. In comparison with the FA-modified PTX/DNA co-loaded liposomes (FA/PPD), HA/FA/PPD showed significant superiority in protecting the nanoparticles from aggregation in the presence of plasma and degradation by DNase I. Moreover, HA/FA/PPD could also significantly improve the transfection efficiency and cellular internalization rates on B16 cells comparing to that of FA/PPD (p < 0.05) and PPD (p < 0.01), demonstrating the great advantages of dual targeting properties. Furthermore, fluorescence microscope and flow cytometry results showed that PTX and DNA could be effectively co-delivered into the same tumor cell via HA/FA/PPD, contributing to PTX/DNA combination cancer treatment. In conclusion, the obtained HA/FA/PPD in the study could effectively target tumor cells, enhance transfection efficiency and subsequently achieve the co-delivery of PTX and DNA, displaying great potential for optimal combination therapy. View Full-Text
Keywords: hyaluronic acid; folate; biomimetic liposomes; paclitaxel; DNA; co-delivery hyaluronic acid; folate; biomimetic liposomes; paclitaxel; DNA; co-delivery
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MDPI and ACS Style

Liu, G.-X.; Fang, G.-Q.; Xu, W. Dual Targeting Biomimetic Liposomes for Paclitaxel/DNA Combination Cancer Treatment. Int. J. Mol. Sci. 2014, 15, 15287-15303.

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