Polydatin Attenuates Hypoxic Pulmonary Hypertension and Reverses Remodeling through Protein Kinase C Mechanisms
Abstract
:1. Introduction
2. Results and Discussion
2.1. Hemodynamics during Hypopiesia and Hypoxia
2.2. Polydatin Attenuates Pulmonary Artery Remodeling and Right Ventricular Hypertrophy
2.3. Effects of Polydatin on Pulmonary Artery Morphology
2.4. Polydatin Reverses Pulmonary Artery Remodeling
2.5. Effects of Polydatin on NO, Ang II and ET
2.6. Effect of Polydatin Is PKC-Dependent
3. Experimental Section
3.1. Reagents and Animals
3.2. Animal Groups and Models
3.3. Measurement of Hemodynamics and Right Ventricular Hypertrophy
3.4. Pulmonary Artery Histology
3.5. Forced Activation of PKC
3.6. Serum NO, ET and Ang II Assays
3.7. Lung Tissue NO, ET and Ang II Assays
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
References
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mPAP (mmHg) | mCAP (mmHg) | |
---|---|---|
control | 18.74 ± 1.74 | 138.05 ± 3.55 |
hypoxia | 32.93 ± 3.08 * | 140.15 ± 5.81 |
silaenafil | 26.08 ± 3.93 ** | 138.20 ± 2.77 |
5 mg/kg PD | 30.34 ± 2.19 | 139.48 ± 4.27 |
10 mg/kg PD | 27.71 ± 2.61 ** | 138.72 ± 3.22 |
20 mg/kg PD | 25.21 ± 2.40 ** | 137.67 ± 4.53 |
MT% | MA% | RV/(LV + S)% | RV/BW (mg/g) | |
---|---|---|---|---|
control | 31.63 ± 2.66 | 43.54 ± 3.17 | 22.20 ± 1.21 | 0.56 ± 0.08 |
hypoxia | 50.72 ± 4.50 * | 72.99 ± 4.47 * | 37.67 ± 2.57 * | 0.92 ± 0.14 * |
silaenafil | 39.28 ± 5.26 ** | 49.84 ± 6.34 ** | 25.57 ± 2.57 ** | 0.63 ± 0.13 ** |
5 mg/kg PD | 46.27 ± 3.88 | 68.85 ± 3.26 | 34.23 ± 1.92 ** | 0.80 ± 0.17 |
10 mg/kg PD | 45.01 ± 4.25 ** | 58.85 ± 4.74 ** | 30.63 ± 1.44 ** | 0.75 ± 0.13 ** |
20 mg/kg PD | 40.75 ± 4.38 ** | 55.27 ± 3.41 ** | 27.87 ± 1.48 ** | 0.73 ± 0.12 ** |
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Miao, Q.; Shi, X.-P.; Ye, M.-X.; Zhang, J.; Miao, S.; Wang, S.-W.; Li, B.; Jiang, X.-X.; Zhang, S.; Hu, N.; et al. Polydatin Attenuates Hypoxic Pulmonary Hypertension and Reverses Remodeling through Protein Kinase C Mechanisms. Int. J. Mol. Sci. 2012, 13, 7776-7787. https://doi.org/10.3390/ijms13067776
Miao Q, Shi X-P, Ye M-X, Zhang J, Miao S, Wang S-W, Li B, Jiang X-X, Zhang S, Hu N, et al. Polydatin Attenuates Hypoxic Pulmonary Hypertension and Reverses Remodeling through Protein Kinase C Mechanisms. International Journal of Molecular Sciences. 2012; 13(6):7776-7787. https://doi.org/10.3390/ijms13067776
Chicago/Turabian StyleMiao, Qing, Xiao-Peng Shi, Ming-Xiang Ye, Jin Zhang, Shan Miao, Si-Wang Wang, Bo Li, Xiu-Xiu Jiang, Song Zhang, Nan Hu, and et al. 2012. "Polydatin Attenuates Hypoxic Pulmonary Hypertension and Reverses Remodeling through Protein Kinase C Mechanisms" International Journal of Molecular Sciences 13, no. 6: 7776-7787. https://doi.org/10.3390/ijms13067776