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Article

Genetic Exchange of Multidrug Efflux Pumps among Two Enterobacterial Species with Distinctive Ecological Niches

by
Nehaya Al-Karablieh
,
Helge Weingart
and
Matthias S. Ullrich
*
School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2009, 10(2), 629-645; https://doi.org/10.3390/ijms10020629
Submission received: 7 January 2009 / Revised: 14 February 2009 / Accepted: 17 February 2009 / Published: 19 February 2009
(This article belongs to the Special Issue Antimicrobial Agents)
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Abstract

AcrAB-TolC is the major multidrug efflux system in Enterobacteriaceae recognizing structurally unrelated molecules including antibiotics, dyes, and detergents. Additionally, in Escherichia coli it mediates resistance to bile salts. In the plant pathogen Erwinia amylovora AcrAB-TolC is required for virulence and phytoalexin resistance. Exchange analysis of AcrAB-TolC was conducted by complementing mutants of both species defective in acrB or tolC with alleles from either species. The acrB and tolC mutants exhibited increased susceptibility profiles for 24 different antibiotics. All mutants were complemented with acrAB or tolC, respectively, regardless of the taxonomic origin of the alleles. Importantly, complementation of E. amylovora mutants with respective E. coli genes restored virulence on apple plants. It was concluded that AcrAB and TolC of both species could interact and that these interactions did not yield in altered functions despite the divergent ecological niches, to which E. coli and E. amylovora have adopted.
Keywords: Erwinia amylovora; Escherichia coli; TolC; AcrAB; multidrug efflux Erwinia amylovora; Escherichia coli; TolC; AcrAB; multidrug efflux

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MDPI and ACS Style

Al-Karablieh, N.; Weingart, H.; Ullrich, M.S. Genetic Exchange of Multidrug Efflux Pumps among Two Enterobacterial Species with Distinctive Ecological Niches. Int. J. Mol. Sci. 2009, 10, 629-645. https://doi.org/10.3390/ijms10020629

AMA Style

Al-Karablieh N, Weingart H, Ullrich MS. Genetic Exchange of Multidrug Efflux Pumps among Two Enterobacterial Species with Distinctive Ecological Niches. International Journal of Molecular Sciences. 2009; 10(2):629-645. https://doi.org/10.3390/ijms10020629

Chicago/Turabian Style

Al-Karablieh, Nehaya, Helge Weingart, and Matthias S. Ullrich. 2009. "Genetic Exchange of Multidrug Efflux Pumps among Two Enterobacterial Species with Distinctive Ecological Niches" International Journal of Molecular Sciences 10, no. 2: 629-645. https://doi.org/10.3390/ijms10020629

APA Style

Al-Karablieh, N., Weingart, H., & Ullrich, M. S. (2009). Genetic Exchange of Multidrug Efflux Pumps among Two Enterobacterial Species with Distinctive Ecological Niches. International Journal of Molecular Sciences, 10(2), 629-645. https://doi.org/10.3390/ijms10020629

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