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Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays

1
Laboratory of Organic Chemistry, Faculty of Pharmacy and Department of Immunology, Medical University, 1 Banacha St., Warsaw, PL-02097, Poland
2
Institute of Chemistry, University of Podlasie, 54 Trzeciego Maja St., Siedlce, PL-08-110, Poland
3
Department of Immunology, Medical University, 2 Oczki St., Warsaw, PL-00-052, Poland
*
Author to whom correspondence should be addressed.
Molecules 2002, 7(12), 896-901; https://doi.org/10.3390/71200896
Received: 25 October 2002 / Accepted: 4 December 2002 / Published: 31 December 2002
A fluorimetric assay of aldehyde dehydrogenase isozymes, based on naphthaldehyde oxidation, is compared with Western Blotting analysis on several clinical samples obtained from surgery. The comparison reveals qualitatively good correlation of ALDH1A1 isozyme detection with two methods and somewhat worse on ALDH3A1 assay. View Full-Text
Keywords: Aldehyde dehydrogenase; isozymes; western blotting; fluorimetric assays Aldehyde dehydrogenase; isozymes; western blotting; fluorimetric assays
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Wroczynski, P.; Wierzchowski, J.; Golab, J.; Blazejczyk, M.; Borecka, D. Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays. Molecules 2002, 7, 896-901.

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