Cinnamomum japonicum Siebold Branch Extracts Attenuate NO and ROS Production via the Inhibition of p38 and JNK Phosphorylation
Abstract
:1. Introduction
2. Results
2.1. Antioxidative Activities and Phenolic Contents of CJB Extracts
2.2. Identification of CJB3 Components Using HPLC
2.3. The Antioxidative Effect of CJB3 on LPS-Activated RAW264.7 Cells
2.4. The Suppressing Effect of CJB3 on Nitric Oxide Synthase
2.5. Effect of CJB3 on LPS-Mediated Production of Proinflammatory Cytokines
2.6. CJB3-Mediated Inhibition of LPS-Induced Phosphorylation of p38, JNK and c-Jun
3. Discussion
4. Material and Methods
4.1. Chemical Extracts of Cinnamomum japonicum Sieb Branch
4.2. Antioxidative Activities and Phenolic Contents
4.2.1. DPPH Radical Scavenging Activity
4.2.2. ABTS Radical-Scavenging Activity
4.2.3. Total Phenolic and Flavonoid Contents
4.3. High-Performance Liquid Chromatography with Diode-Array Detection (HPLC–DAD) Analysis
4.4. Cell Culture
4.5. Assay of Nitrite Production
4.6. Cytotoxicity Assay
4.7. ROS Generation Assay of Bambusae Caulis in Liquamen
4.8. Immunoblot Analysis
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Sample | DPPH IC50 (μg/mL) | ABTS IC50 (μg/mL) | TPC | TFC |
---|---|---|---|---|
GAE mg/g | QUE mg/g | |||
CJB1 | 643.07 ± 52.19 | 306.48 ± 2.79 | 12.39 ± 0.68 | 1.94 ± 5.80 |
CJB2 | 793.32 ± 15.37 | 168.45 ± 1.15 | 19.21 ± 4.71 | 16.37 ± 1.04 |
CJB3 | 144.72 ± 4.58 | 35.08 ± 0.06 | 50.65 ± 1.64 | 143.28 ± 6.34 |
CJB4 | 243.02 ± 7.62 | 67.10 ± 0.71 | 30.60 ± 0.72 | 62.08 ± 1.04 |
CJB5 | 827.06 ± 16.51 | 313.18 ± 2.28 | 10.92 ± 0.13 | 4.94 ± 1.80 |
Catechin | 73.63 ± 1.86 | 17.18 ± 0.87 | - | - |
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Kim, J.M.; Choi, M.-H.; Yang, J.H. Cinnamomum japonicum Siebold Branch Extracts Attenuate NO and ROS Production via the Inhibition of p38 and JNK Phosphorylation. Molecules 2023, 28, 1974. https://doi.org/10.3390/molecules28041974
Kim JM, Choi M-H, Yang JH. Cinnamomum japonicum Siebold Branch Extracts Attenuate NO and ROS Production via the Inhibition of p38 and JNK Phosphorylation. Molecules. 2023; 28(4):1974. https://doi.org/10.3390/molecules28041974
Chicago/Turabian StyleKim, Jae Min, Moon-Hee Choi, and Ji Hye Yang. 2023. "Cinnamomum japonicum Siebold Branch Extracts Attenuate NO and ROS Production via the Inhibition of p38 and JNK Phosphorylation" Molecules 28, no. 4: 1974. https://doi.org/10.3390/molecules28041974