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Article

Enhanced Activity by Genetic Complementarity: Heterologous Secretion of Clostridial Cellulases by Bacillus licheniformis and Bacillus velezensis

1
Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
2
Institute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
*
Author to whom correspondence should be addressed.
Academic Editor: Stefan Janecek
Molecules 2021, 26(18), 5625; https://doi.org/10.3390/molecules26185625
Received: 13 August 2021 / Revised: 11 September 2021 / Accepted: 13 September 2021 / Published: 16 September 2021
(This article belongs to the Special Issue Recent Advances in Carbohydrate-Active Enzymes)
To adapt to various ecological niches, the members of genus Bacillus display a wide spectrum of glycoside hydrolases (GH) responsible for the hydrolysis of cellulose and lignocellulose. Being abundant and renewable, cellulose-containing plant biomass may be applied as a substrate in second-generation biotechnologies for the production of platform chemicals. The present study aims to enhance the natural cellulase activity of two promising 2,3-butanediol (2,3-BD) producers, Bacillus licheniformis 24 and B. velezensis 5RB, by cloning and heterologous expression of cel8A and cel48S genes of Acetivibrio thermocellus. In B. licheniformis, the endocellulase Cel8A (GH8) was cloned to supplement the action of CelA (GH9), while in B. velezensis, the cellobiohydrolase Cel48S (GH48) successfully complemented the activity of endo-cellulase EglS (GH5). The expression of the natural and heterologous cellulase genes in both hosts was demonstrated by reverse-transcription PCR. The secretion of clostridial cellulases was additionally enhanced by enzyme fusion to the subtilisin-like signal peptide, reaching a significant increase in the cellulase activity of the cell-free supernatants. The results presented are the first to reveal the possibility of genetic complementation for enhancement of cellulase activity in bacilli, thus opening the prospect for genetic improvement of strains with an important biotechnological application. View Full-Text
Keywords: cellulose; 2,3-butanediol; Bacillus licheniformis; Bacillus velezensis; cellulase; heterologous expression; signal peptide; RT-PCR; pBE-S shuttle vector cellulose; 2,3-butanediol; Bacillus licheniformis; Bacillus velezensis; cellulase; heterologous expression; signal peptide; RT-PCR; pBE-S shuttle vector
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MDPI and ACS Style

Arsov, A.; Petrov, K.; Petrova, P. Enhanced Activity by Genetic Complementarity: Heterologous Secretion of Clostridial Cellulases by Bacillus licheniformis and Bacillus velezensis. Molecules 2021, 26, 5625. https://doi.org/10.3390/molecules26185625

AMA Style

Arsov A, Petrov K, Petrova P. Enhanced Activity by Genetic Complementarity: Heterologous Secretion of Clostridial Cellulases by Bacillus licheniformis and Bacillus velezensis. Molecules. 2021; 26(18):5625. https://doi.org/10.3390/molecules26185625

Chicago/Turabian Style

Arsov, Alexander, Kaloyan Petrov, and Penka Petrova. 2021. "Enhanced Activity by Genetic Complementarity: Heterologous Secretion of Clostridial Cellulases by Bacillus licheniformis and Bacillus velezensis" Molecules 26, no. 18: 5625. https://doi.org/10.3390/molecules26185625

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