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Article

Vectorizing Pro-Insecticide: Influence of Linker Length on Insecticidal Activity and Phloem Mobility of New Tralopyril Derivatives

by
Tian Xing Li
1,
Yao Chen
2,
Hui Fang Liu
2,
Chi Yu Ma
2 and
Wen Yang
2,*
1
College of Tea Science, Guizhou University, Guiyang 550025, China
2
Guizhou Tea Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(15), 4570; https://doi.org/10.3390/molecules26154570
Submission received: 21 June 2021 / Revised: 22 July 2021 / Accepted: 23 July 2021 / Published: 28 July 2021
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Abstract

:
To improve the proinsecticidal activity and phloem mobility of amino acid–tralopyril conjugates further, nine conjugates were designed and synthesized by introducing glutamic acid to tralopyril, and the length of the linker between glutamic acid and tralopyril ranged from 2 atoms to 10 atoms. The results of insecticidal activity against the third-instar larvae of P. xylostella showed that conjugates 42, 43, 44,and 45 (straight-chain containing 2–5 atoms) exhibited good insecticidal activity, and their LC50 values were 0.2397 ± 0.0366, 0.4413 ± 0.0647, 0.4400 ± 0.0624, and 0.4602 ± 0.0655 mM, respectively. The concentrations of conjugates 43–45 were higher than that of conjugate 42 in the phloem sap at 2 h, and conjugate 43 showed the highest concentration. The introduction of glutamic acid can improve phloem mobility. The in vivo metabolism of conjugates 42 and 43 was investigated in P. xylostella, and the parent compound tralopyril was detected at concentrations of 0.5950 and 0.3172 nmol/kg, respectively. According to the above results, conjugates 42 and 43 were potential phloem mobile pro-insecticide candidates.

1. Introduction

The phloem systemicity of pesticides is an important characteristic for the expression of their biological activities [1,2]. Insecticides with phloem mobility are preferred for controlling pests hidden on unpredictable nonexposed plant parts, such as growing tips, roots, inside leaf deformations, and galls [3]. However, few existing insecticides have good phloem mobility. Currently, only spirotetramat is an ambimobile systemic (phloem and xylem systemic) insecticide in plants, which can be used to control hidden and soil living sucking pests, such as aphids and whiteflies [3].
The carrier-mediated strategy has been used to develop novel phloem mobile insecticides [1]. Several attempts have been made to achieve phloem-mobile insecticides by introducing a carboxyl group, amino acid, or sugar to the parent compounds of existing non-phloem-mobile pesticides [2,4,5]. Pioneering work was conducted to achieve phloem-mobile pronematicide by introducing glucuronic acid moiety to oxamyl [4]. One vectorization strategy of agrochemicals has been established by introducing carboxyl group, amino acid, and sugar to phenylpyrrole fungicide [6,7,8]. Plant amino acid and sugar transporters have been reported to interact with pesticide conjugates and enhance their phloem translocation [9,10].
Tralopyril (Figure 1) is a biocide optimized from dioxapyrrolomycin that shows excellent insecticidal activity [11,12]. However, this compound exhibits phytotoxic effects. Chlorfenapyr is the first commercial proinsecticide developed by introducing ethoxymethyl group into the pyrrole N of tralopyril [11,12,13,14]. Tralopyril is a nonsystemic insecticide without phloem mobility. To address the problems of systemicity and phytotoxicity, a strategy of vectorizing the pro-insecticide of tralopyril was proposed in our research group. The six new conjugates were designed and synthesized by introducing glucose, methyl glucuronate, and glucuronic acid moieties into tralopyril. The phloem mobility results demonstrated that our strategy can improve the phloem mobility of tralopyril [15,16].
Our previous results demonstrated that introducing glutamic acid moieties can improve the phloem mobility of tralopyril [16]. However, the structure–activity relationship of tralopyril conjugates has not yet been investigated, especially for the linker structure. In previous studies, the linker structure between the nutrient promoiety and active ingredient influence the bioactivity and phloem mobility of the conjugates [8,17]. Hence, the aim of the present study is to elucidate the structure–activity relationship for the linker between amino acid promoiety and tralopyril. (I) Nine tralopyril conjugates containing glutamic acid with different linker lengths were synthesized. (II) The insecticidal activity of the conjugates against the third-instar larvae of P. xylostella was evaluated using a poison fodder method. (III) Phloem mobility test in castor bean seedlings was performed. (IV) The metabolism of conjugates 42 and 43 in P. xylostella was investigated. This study provides a fundamental understanding of the influence of different linker lengths on the insecticidal activity and phloem mobility of new tralopyril derivatives.

2. Materials and Methods

2.1. Synthesis

2.1.1. General Information for Synthesis

Solvents were of analytical grade and were dried according to the methods of the Purification of Laboratory Chemicals, Fifth Edition. Reagents were purchased from a commercial company. 1H NMR and 13C NMR spectra were obtained on a Varian INOVA-400 instrument. Chemical shifts were expressed in δ(ppm) values, with TMS as an internal standard. Analytical thin-layer chromatography (TLC) was performed on silicagel GF254. Silica gel (100–200 and 200–300 mesh, Qingdao Marine Chemical Ltd., Qingdao, China) was used for column chromatography.

2.1.2. Synthesis of Compound 4

Synthesis of the title compound was accomplished according to a previously reported procedure [16]. NaH (60% in oil, 1 g, 25 mmol) was added to the solution of tralopyril (3.5 g, 10 mmol) in a mixture solvent of dry THF (30 mL) and bromochloromethane (12.94 g, 100 mmol, 5 equiv). The mixture was then stirred at room temperature for 2 h. Propargyl alcohol (10 mmol) in THF (10 mL) was added dropwise and stirred at 65 °C for 48 h. The reaction mixture was quenched by adding ice water, and the resultant mixture was extracted with ethyl acetate (20 mL × 3). The combined organic layers were washed with aqueous sodium hydrogen carbonate and brine, dried with sodium sulfate, filtered, and evaporated in vacuo. The residues were purified by column chromatography to obtain the desired product 4 as a white solid with 63% yield. 1H NMR (400 MHz, DMSO-d6) δ 7.69 (m, 4H, Ar-H), 5.38 (s, 2H), 4.05 (d, J = 2.4 Hz, 2H), 3.37 (t, J = 2.4 Hz, 1H). 13C NMR (100 MHz, DMSO-d6) δ 144.60, 135.88, 132.04 × 2, 129.32 × 2, 125.26, 121.31, 118.66, 113.61, 103.44, 97.96, 78.10, 77.96, 74.77, 55.65. API-ES (m/z): 441. 00 [M + Na]+.

2.1.3. General Procedures for Compounds 14–22

Synthesis of compounds 14–22 followed the procedure previously reported by Lu and Bittman [16,18]. In a 20 mL flask, compounds 5–13 (10 mmol), NaN3 (0.85 g, 13.2 mmol), and n-Bu4NBr (80 mg, 0.25 mmol) were mixed and stirred for 15 h at 110 °C. After the mixture had cooled, the product was taken up with CH2Cl2 (20 mL), and the precipitate (consisting of NaBr, unreacted NaN3, and phase-transfer catalyst) was removed by filtration. The salt was washed with CH2Cl2 (20 mL). Evaporation of the solvents resulted in a yellow residue that was purified by distillation at 35 °C to yield compounds 14–22 as a colorless liquid.

2.1.4. General Procedures for Compounds 23–31

Synthesis of the title compound was accomplished according to a previously reported procedure [16]. Compounds 23–31 were synthesized using this procedure. Tralopyril derivative 4 (10 mmol) and crude azide 14–22 (10 mmol) were dissolved in 20 mL DMF. The reaction was initiated by the addition of CuSO4·5H2O (4 mmol) and sodium ascorbate (8 mmol) in 5 mL distilled water. The mixture was stirred at 60 °C until TLC indicated the disappearance of the starting materials. The mixture was poured into distilled water (20 mL), and the product was extracted three times with ethyl acetate (15 mL × 3). The organic layer was dried with Na2SO4 and filtered. The solvent was removed under reduced pressure. The residues were purified by column chromatography to obtain the compounds 23–31.
Compound 23: 1H NMR (400 MHz, DMSO-d6) δ 7.89 (s, 1H), 7.64~7.67 (m, 4H), 5.38 (s, 2H), 5.02 (s, 1H), 4.41 (s, 2H), 4.36 (t, 2H, J = 3.6 Hz), 3.74 (s, 2H). 13C NMR (100 MHz, DMSO-d6) δ 144.35, 141.77, 135.75, 131.80, 129.16 × 2, 125.20, 124.52 × 2, 120.77, 119.05, 113.74, 103.13, 97.82, 75.28, 61.30, 59.72, 52.02. API-ES (m/z): 528.3 [M + Na]+.
Compound 24: 1H NMR (400 MHz, DMSO-d6) δ 7.91 (s, 1H), 7.65 (m, 4H), 5.37 (s, 2H), 5.02 (s, 1H), 4.43 (s, 2H), 4.38 (t, 2H, J = 4.8 Hz), 3.39 (t, 2H, J = 4.0 Hz), 1.90~1.95 (m, 2H). 13C NMR (100 MHz, DMSO-d6) δ 144.35, 141.92, 135.81, 131.79, 129.17 × 2, 125.20, 124.13 × 2, 120.78, 118.99, 113.48, 103.17, 97.87, 75.23, 61.33, 57.33, 46.57 32.88. API-ES (m/z): 542.3 [M + Na]+.
Compound 25: 1H NMR (400 MHz, DMSO-d6) δ 7.91 (s, 1H), 7.65 (m, 4H), 5.37 (s, 2H), 5.34 (s, 1H), 4.43 (d, 2H, J = 4.4 Hz), 4.34~4.36 (m, 2H), 3.42 (t, 2H, J = 4.0 Hz), 1.80~1.83 (m, 2H), 1.34~1.38 (m, 2H). 13C NMR (100 MHz, DMSO-d6) δ 144.33, 142.00, 135.77, 131.78, 129.14 × 2, 125.19, 124.03 × 2, 120.75, 118.97, 113.45, 103.13, 97.85, 75.24, 61.30, 59.90, 49.20, 29.10, 26.65. API-ES (m/z): 556.3 [M + Na]+.
Compound 26: 1H NMR (400 MHz, DMSO-d6) δ 7.90 (s, 1H), 7.62~7.65 (m, 4H), 5.37 (s, 2H), 4.48 (s, 1H), 4.44 (s, 2H), 4.30 (t, 2H, J = 4.8 Hz), 3.38 (t, 2H, J = 4.4 Hz), 1.75~1.80 (m, 2H), 1.42~1.46 (m, 2H), 1.21~1.27 (m, 2H). 13C NMR (100 MHz, DMSO-d6) δ 144.33, 141.89, 135.79, 131.78, 129.15 × 2, 125.19, 123.91 × 2, 120.76, 118.98, 113.46, 103.13, 97.86, 75.18, 61.32, 60.38, 49.32, 31.71, 29.58, 22.40. API-ES (m/z): 570.2 [M + Na]+.
Compound 27: 1H NMR (400 MHz, DMSO-d6) δ 7.91 (s, 1H), 7.62~7.66 (m, 4H), 5.38 (s, 2H), 4.47 (s, 2H), 4.37 (s, 1H), 4.31 (t, 2H, J = 4.8 Hz), 3.39 (t, 2H, J = 3.6 Hz), 1.76~1.81 (m, 2H), 1.38~1.43 (m, 2H), 1.29~1.34 (m, 2H), 1.19~1.24 (m, 2H). 13C NMR (100 MHz, DMSO-d6) δ 144.32, 141.93, 135.87, 131.73, 129.14 × 2, 125.18, 123.89 × 2, 120.78, 118.99, 113.41, 103.11, 97.95, 75.14, 61.36, 60.60, 49.31, 32.25, 29.76, 25.69, 24.87. API-ES (m/z): 584.3 [M + Na]+.
Compound 28: 1H NMR (400 MHz, DMSO-d6) δ 7.90 (s, 1H), 7.62~7.66 (m, 4H), 5.37 (s, 2H), 4.46 (s, 2H), 4.34 (s, 1H), 4.30 (t, 2H, J = 4.8 Hz), 3.38 (t, 2H, J = 4.0 Hz), 1.74~1.79 (m, 2H), 1.37~1.41 (m, 2H), 1.23~1.28 (m, 4H), 1.17~1.22 (m, 2H). 13C NMR (100 MHz, DMSO-d6) δ 144.30, 141.89, 135.81, 131.74, 129.13 × 2, 125.18, 123.88 × 2, 120.76, 118.97, 113.41, 103.11, 97.89, 75.14, 61.32, 60.64, 49.27, 32.34, 29.65, 28.23, 25.79, 25.27. API-ES (m/z): 598.3 [M + Na]+.
Compound 29: 1H NMR (400 MHz, DMSO-d6) δ 7.91 (s, 1H), 7.62~7.66 (m, 4H), 5.36 (s, 2H), 4.46 (s, 2H), 4.33 (s, 1H), 4.30 (t, 2H, J = 4.4 Hz), 3.38 (t, 2H, J = 4.4 Hz), 1.74~1.79 (m, 2H), 1.37~1.42 (m, 2H), 1.18~1.27 (m, 8H). 13C NMR (100 MHz, DMSO-d6) δ 144.29, 141.89, 135.81, 131.74, 129.12 × 2, 125.18, 123.88 × 2, 120.75, 118.97, 113.39, 103.11, 97.90, 75.13, 61.32, 60.68, 49.27, 32.44, 29.67, 28.73, 28.40, 25.74, 25.37. API-ES (m/z): 612.3 [M + Na]+.
Compound 30: 1H NMR (400 MHz, DMSO-d6) δ 7.90 (s, 1H), 7.62~7.66 (m, 4H), 5.35 (s, 2H), 4.43 (s, 2H), 4.34 (s, 1H), 4.29 (t, 2H, J = 4.8 Hz), 3.38 (m, 2H), 1.73~1.78 (m, 2H), 1.37~1.40 (m, 2H), 1.22~1.24 (m, 8H), 1.16~1.90 (m, 2H). 13C NMR (100 MHz, DMSO-d6) δ 144.29, 141.87, 135.76, 131.77, 129.13 × 2, 125.18, 123.90 × 2, 120.75, 118.96, 113.42, 103.13, 97.83, 75.15, 61.29, 60.66, 49.23, 32.45, 29.64, 29.10, 28.83, 28.28, 25.71, 25.40. API-ES (m/z): 626.5 [M + Na]+.
Compound 31: 1H NMR (400 MHz, DMSO-d6) δ 7.90 (s, 1H), 7.63~7.66 (m, 4H), 5.34 (s, 2H), 4.42 (s, 2H), 4.47 (s, 1H), 4.29 (t, 2H, J = 4.8 Hz), 3.36 (t, 2H, J = 4.4 Hz), 1.72~1.77 (m, 2H), 1.36~1.40 (m, 2H), 1.20~1.24 (m, 10H), 1.16~1.19 (m, 2H). 13C NMR (100 MHz, DMSO-d6) δ 144.30, 141.87, 135.74, 131.78, 129.14 × 2, 125.18, 123.92 × 2, 120.75, 118.96, 113.45, 103.12, 97.81, 75.15, 61.28, 60.65, 49.21, 32.46, 29.62, 28.91, 28.84, 28.75, 28.28, 25.70, 25.41. API-ES (m/z): 640.2 [M + Na]+.

2.1.5. Synthesis of Compounds 33–41

Synthesis of the title compound was accomplished according to a previously reported procedure [16]. EDC (0.747 g, 3.90 mmol) and DMAP (0.293 g, 2.4 mmol) were added to a solution of Boc-Glu-OH (1.00 g, 3.90 mmol) in CH2Cl2 (5 mL), followed by 23–31 (3 mmol). The mixture was stirred at rt for 6 h, followed by extraction with H2O (3 × 5 mL) and drying with MgSO4. After the drying agent was filtered, the solvent was removed under reduced pressure. The residues were purified by column chromatography to obtain compounds 33–41.
Compound 33: 1H NMR (400 MHz, DMSO-d6): δ 7.99 (s, 1H), 7.63~7.65 (m, 4H), 7.11 (d, 1H, J = 5.2 Hz), 5.38 (s, 2H), 4.62 (t, 2H, J = 3.6 Hz), 4.45 (s, 2H), 4.40 (t, 2H, J = 3.6 Hz), 3.80~3.84 (m, 1H), 2.29~2.37 (m, 2H), 1.34~1.39 (m, 20H). 13C NMR (100 MHz, DMSO-d6): δ 171.73, 171.18, 155.43, 144.32, 142.11, 135.81, 131.75, 129.14, 125.17, 124.51, 120.75, 119.41, 119.16, 118.96, 113.45, 103.09, 97.91, 80.29, 78.01, 75.20, 62.26, 59.62, 53.35, 48.42, 29.76, 27.99 × 2, 27.75, 27.43 × 3, 25.67. API-ES (m/z): 813.3 [M + Na]+.
Compound 34: 1H NMR (400 MHz, DMSO-d6): δ 7.95 (s, 1H), 7.63~7.66 (m, 4H), 7.13 (d, 1H, J = 5.2 Hz), 5.37 (s, 2H), 4.44 (s, 2H), 4.42 (t, 2H, J = 4.4 Hz), 4.00~4.04 (m, 2H), 3.84~3.88 (m, 1H), 2.35~2.38 (m, 2H), 2.10~2.14 (m, 2H), 1.34~1.39 (m, 20H). 13C NMR (100 MHz, DMSO-d6): δ 172.00, 171.25, 155.46, 144.30, 142.00, 135.78, 131.76, 129.12, 125.17, 124.11, 120.74, 119.38, 119.13, 118.95, 113.34, 103.06, 97.88, 80.30, 78.00, 75.17, 61.27, 60.82, 53.37, 46.33, 29.84, 28.85, 27.99 × 2, 27.75, 27.46 × 3, 25.79. API-ES (m/z): 827.5 [M + Na]+.
Compound 35: 1H NMR (400 MHz, DMSO-d6): δ 7.92 (s, 1H), 7.62~7.65 (m, 4H), 7.13 (d, 1H, J = 5.2 Hz), 5.36 (s, 2H), 4.44 (s, 2H), 4.35 (t, 2H, J = 4.4 Hz), 4.01~4.04 (m, 2H), 3.83~3.87 (m, 1H), 2.35~2.40 (m, 2H), 1.75~1.87 (m, 4H), 1.50~1.54 (m, 2H), 1.34~1.41 (m, 20H). 13C NMR (100 MHz, DMSO-d6): δ 172.07, 171.27, 155.44, 144.29, 141.93, 135.76, 131.76, 129.12, 125.17, 123.94, 120.74, 119.37, 119.12, 118.95, 113.35, 103.07, 97.86, 80.26, 77.98, 75.16, 63.08, 61.29, 53.36, 48.81, 29.87, 28.00, 27.76, 27.45 × 3, 26.30, 25.82, 25.03. API-ES (m/z): 841.7 [M + Na]+.
Compound 36: 1H NMR (400 MHz, DMSO-d6): δ 7.91 (s, 1H), 7.62~7.64 (m, 4H), 7.12 (d, 1H, J = 5.2 Hz), 5.37 (s, 2H), 4.45 (s, 2H), 4.32 (t, 2H, J = 4.4 Hz), 3.98~4.04 (m, 2H), 2.34~2.39 (m, 2H), 1.79~1.82 (m, 3H), 1.58~1.60 (m, 2H), 1.35~1.39 (m, 20H), 1.24~1.29 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 172.05, 171.27, 155.44, 144.27, 141.89, 135.77, 131.74, 129.11, 125.17, 123.86, 120.74, 119.40, 119.14, 118.95, 113.32, 103.06, 97.88, 80.25, 77.97, 75.13, 63.45, 61.29, 53.37, 49.04, 30.59, 29.88, 29.22, 27.99 × 2, 27.45, 27.44, 27.37, 27.31, 27.31, 25.85, 22.19. API-ES (m/z): 855.7 [M + Na]+.
Compound 37: 1H NMR (400 MHz, DMSO-d6): δ 7.90 (s, 1H), 7.63~7.65 (m, 4H), 7.12 (d, 1H, J = 5.2 Hz), 5.36 (s, 2H), 4.44 (s, 2H), 4.30 (t, 2H, J = 4.8 Hz), 3.98~4.00 (m, 2H), 2.34~2.38 (m, 2H), 1.74~1.80 (m, 3H), 1.52~1.56 (m, 2H), 1.40~1.43 (m, 6H), 1.37~1.38 (m, 14H), 1.33~1.34 (m, 2H), 1.20~1.25 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 172.06, 171.26, 155.42, 144.26, 141.85, 135.74, 131.74, 129.10, 125.16, 123.85, 120.72, 119.36, 119.11, 118.94, 113.33, 103.06, 97.84, 80.23, 77.94, 75.13, 63.59, 61.29, 53.34, 49.11, 30.59, 29.89, 29.50, 27.99, 27.82, 27.75, 27.45, 27.35, 27.32, 25.84, 25.33, 24.65. API-ES (m/z): 869.7 [M + Na]+.
Compound 38: 1H NMR (400 MHz, DMSO-d6): δ 7.90 (s, 1H), 7.63~7.66 (m, 4H), 7.12 (d, 1H, J = 5.2 Hz), 5.36 (s, 2H), 4.44 (s, 2H), 4.30 (t, 2H, J = 4.4 Hz), 3.98~4.00 (m, 2H), 2.33~2.38 (m, 2H), 1.74~1.80 (m, 3H), 1.53~1.55 (m, 2H), 1.38~1.43 (m, 20H), 1.27~1.29 (m, 4H), 1.20~1.22 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 172.06, 171.26, 155.41, 144.25, 141.85, 135.75, 131.73, 129.10, 125.16, 123.83, 120.72, 119.38, 119.13, 118.93, 113.31, 103.04, 97.86, 80.21, 77.94, 75.12, 63.68, 61.29, 53.34, 49.17, 30.59, 29.89, 29.55, 27.99, 27.94, 27.91, 27.74, 27.44, 27.34, 27.31, 25.86, 25.60, 25.10. API-ES (m/z): 883.5 [M + Na]+.
Compound 39: 1H NMR (400 MHz, DMSO-d6): δ 7.90 (s, 1H), 7.63~7.66 (m, 4H), 7.12 (d, 1H, J = 5.2 Hz), 5.36 (s, 2H), 4.44 (s, 2H), 4.30 (t, 2H, J = 4.4 Hz), 3.98~4.00 (m, 2H), 2.33~2.38 (m, 2H), 1.75~1.78 (m, 3H), 1.53~1.55 (m, 2H), 1.41~1.43 (m, 20H), 1.26~1.28 (m, 6H), 1.18~1.21 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 172.04, 171.25, 155.40, 144.25, 141.83, 135.73, 131.73, 129.09, 125.16, 123.84, 120.72, 119.36, 119.10, 118.93, 113.31, 103.03, 97.84, 80.21, 77.92, 75.12, 63.68, 61.28, 58.96, 53.33, 49.17, 30.59, 29.88, 29.59, 28.36, 28.16, 27.99, 27.74, 27.44, 27.35, 27.32, 25.85, 25.62, 25.15. API-ES (m/z): 897.5 [M + Na]+.
Compound 40: 1H NMR (400 MHz, DMSO-d6): δ 7.90 (s, 1H), 7.62~7.64 (m, 4H), 7.11 (d, 1H, J = 5.2 Hz), 5.35 (s, 2H), 4.43 (s, 2H), 4.29 (t, 2H, J = 4.4 Hz), 3.98~4.00 (m, 2H), 2.32~2.38 (m, 2H), 1.74~1.78 (m, 3H), 1.52~1.55 (m, 2H), 1.41~1.43 (m, 12H), 1.37~1.38 (m, 14H), 1.25~1.27 (m, 4H). 13C NMR (100 MHz, DMSO-d6): δ 172.05, 171.26, 155.40, 144.26, 141.84, 135.72, 131.74, 129.10, 125.16, 123.85, 120.72, 119.33, 119.08, 118.93, 113.34, 103.05, 97.82, 80.22, 77.93, 75.13, 63.72, 61.27, 58.96, 53.31, 49.18, 30.59, 29.89, 29.59, 28.63, 28.45, 28.20, 27.99, 27.76, 27.46, 27.36, 27.33, 25.84, 25.66, 25.21. API-ES (m/z): 911.5 [M + Na]+.
Compound 41: 1H NMR (400 MHz, DMSO-d6): δ 8.52 (m, 2H), 7.90 (s, 1H), 7.63~7.64 (m, 4H), 5.35 (s, 2H), 4.43 (s, 2H), 4.29 (t, 2H, J = 4.4 Hz), 3.97~4.00 (m, 2H), 2.32~2.38 (m, 2H), 1.73~1.78 (m, 3H), 1.52~1.55 (m, 2H), 1.41~1.43 (m, 10H), 1.37~1.38 (m, 16H), 1.24~1.26 (m, 6H). 13C NMR (100 MHz, DMSO-d6): δ 172.05, 171.26, 155.40, 144.26, 141.84, 135.72, 131.74, 129.10, 125.16, 123.85, 120.72, 119.33, 119.08, 118.93, 113.35, 103.05, 97.82, 80.23, 77.94, 75.13, 63.73, 61.27, 58.96, 53.32, 49.18, 30.60, 29.89, 29.61, 28.72, 28.68, 28.52, 28.26, 28.01, 27.76, 27.46, 27.37, 27.34, 25.84, 25.67, 25.24. API-ES (m/z): 925.7 [M + Na]+.

2.1.6. Synthesis of Conjugates 42–50

Synthesis of the title compound was accomplished according to a previously reported procedure [16]. Compounds 33–41 were added to a mixture solution of CH2Cl2 (3 mL) and TFA (3 mL). The mixture was stirred at rt for 12 h, after which the solvent was removed in vacuo. The residues were purified by column chromatography to obtain conjugates 42–50.
Conjugate 42: 1H NMR (400 MHz, DMSO-d6): δ 8.50 (d, 2H, J = 4.8 Hz), 7.96 (s, 1H), 7.61~7.63 (m, 4H), 5.36 (s, 2H), 4.36~4.41 (m, 4H), 3.98~4.01 (m, 2H), 3.75 (s, 1H), 2.42~2.50 (m, 2H), 1.95~2.10 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ172.32, 170.62, 144.59, 142.35, 141.98, 136.02, 131.95, 129.36, 125.29, 124.88, 120.92, 119.62, 119.37, 119.13, 113.69, 103.32, 98.03, 75.35, 62.45, 61.35, 52.28, 29.80, 28.25. API-ES (m/z): 656.2 [M + Na]+.
Conjugate 43: 1H NMR (400 MHz, DMSO-d6): δ 8.54 (m, 2H), 7.96 (s, 1H), 7.64 (m, 4H), 5.35 (s, 2H), 4.39~4.41 (m, 4H), 3.98 (t, 2H, J = 3.6 Hz), 3.59 (s, 1H), 2.45~2.55 (m, 2H), 2.09 (t, 2H, J = 3.6 Hz), 1.91~2.02 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 172.13, 170.98, 144.45, 142.13, 141.98, 135.86, 131.89, 129.26, 125.25, 124.32, 120.84, 119.44, 119.19, 119.05, 113.57, 103.24, 97.90, 75.30, 61.33, 60.97, 46.36, 29.56, 28.94, 25.73. API-ES (m/z): 670.2 [M + Na]+.
Conjugate 44: 1H NMR (400 MHz, DMSO-d6): δ 8.53 (m, 2H), 7.92 (s, 1H), 7.63~7.65 (m, 4H), 5.35 (s, 2H), 4.35~4.40 (m, 4H), 4.02~4.04 (m, 2H), 3.61 (s, 1H), 2.40~2.45 (m, 2H), 1.97~2.01 (m, 2H), 1.51 (m, 2H), 1.40~1.43 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 171.94, 168.33, 144.36, 142.08, 141.96, 135.74, 131.84, 129.59, 125.21, 124.07, 120.76, 119.30, 119.05, 118.98, 113.49, 103.17, 97.80, 75.26, 63.29, 61.27, 51.59, 28.95, 27.41, 26.30, 25.02. API-ES (m/z): 684.3 [M + Na]+.
Conjugate 45: 1H NMR (400 MHz, DMSO-d6): δ 8.51 (m, 2H), 7.90 (s, 1H), 7.62~7.66 (m, 4H), 5.34 (s, 2H), 4.39~4.41 (m, 2H), 4.30 (t, 2H, J = 4.4 Hz), 3.99 (t, 2H, J = 4.4 Hz), 3.68 (s, 1H), 2.39~2.45 (m, 2H), 1.91~2.03 (m, 2H), 1.75~1.80 (m, 2H), 1.56~1.61 (m, 2H), 1.24~1.27 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 171.96, 170.35, 144.38, 142.07, 141.96, 135.77, 131.86, 129.20, 125.23, 124.01, 120.79, 119.34, 119.08, 119.00, 113.53, 103.19, 97.81, 75.24, 63.76, 61.31, 51.91, 29.41, 29.27, 27.39, 25.53, 22.24. API-ES (m/z): 698.0 [M + Na]+.
Conjugate 46: 1H NMR (400 MHz, DMSO-d6): δ 8.50 (m, 2H), 7.90 (s, 1H), 7.63~7.67 (m, 4H), 5.34 (s, 2H), 4.41 (m, 2H), 4.29 (t, 2H, J = 4.8 Hz), 3.99 (t, 2H, J = 4.4 Hz), 3.69 (s, 1H), 2.40~2.55 (m, 2H), 1.91~2.04 (m, 2H), 1.75~1.78 (m, 2H), 1.51~1.56 (m, 2H), 1.29~1.32 (m, 2H), 1.19~1.23 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 171.92, 170.39, 144.33, 142.02, 141.89, 135.73, 131.82, 129.16, 125.20, 123.96, 120.76, 119.29, 119.04, 118.97, 113.48, 103.16, 97.78, 75.21, 63.84, 61.28, 51.89, 29.50, 29.39, 27.81, 25.53, 25.35, 24.66. API-ES (m/z): 712.5 [M + Na]+.
Conjugate 47: 1H NMR (400 MHz, DMSO-d6): δ 8.51 (m, 2H), 7.90 (s, 1H), 7.63 (m, 4H), 5.34 (s, 2H), 4.41 (m, 2H), 4.28 (t, 2H, J = 4.0 Hz), 3.99 (t, 2H, J = 4.4 Hz), 3.74 (s, 1H), 2.44~2.52 (m, 2H), 1.94~2.02 (m, 2H), 1.73 (m, 2H), 1.53 (m, 2H), 1.27 (m, 4H), 1.18 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 172.00, 170.68, 144.41, 142.07, 141.96, 135.82, 131.88, 129.23, 125.26, 124.06, 120.83, 119.41, 119.16, 119.04, 113.57, 103.23, 97.86, 75.26, 64.03, 61.34, 51.90, 29.62, 29.41, 28.17, 27.97, 25.67, 25.54, 25.17. API-ES (m/z): 726.3 [M + Na]+.
Conjugate 48: 1H NMR (400 MHz, DMSO-d6): δ 8.50 (m, 2H), 7.90 (s, 1H), 7.64 (m, 4H), 5.34 (s, 2H), 4.41 (m, 2H), 4.28 (t, 2H, J = 3.6 Hz), 3.98 (t, 2H, J = 4.4 Hz), 3.71 (s, 1H), 2.43~2.50 (m, 2H), 1.93~2.02 (m, 2H), 1.74 (m, 2H), 1.53 (m, 2H), 1.24 (m, 6H), 1.13~1.16 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 172.26, 170.33, 144.36, 142.02, 141.91, 135.78, 131.84, 129.19, 125.22, 124.01, 120.79, 119.36, 119.11, 118.90, 113.51, 103.17, 97.83, 75.22, 63.88, 61.31, 52.70, 29.70, 29.63, 28.43, 28.22, 28.03, 25.91, 25.68, 25.22. API-ES (m/z): 740.3 [M + Na]+.
Conjugate 49: 1H NMR (400 MHz, DMSO-d6): δ 8.52 (m, 2H), 7.90 (s, 1H), 7.60~7.64 (m, 4H), 5.35 (s, 2H), 4.46 (m, 2H), 4.29 (t, 2H, J = 4.4 Hz), 3.97~3.99 (m, 3H), 2.35~2.41 (m, 2H), 1.87~1.96 (m, 2H), 1.74~1.76 (m, 2H), 1.50~1.56 (m, 4H), 1.40~1.42 (m, 6H), 1.18~1.19 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 172.49, 171.09, 144.28, 142.02, 141.90, 135.89, 131.69, 129.13, 125.17, 123.86, 120.77, 119.58, 119.33, 118.98, 113.30, 103.04, 97.99, 75.10, 63.75, 61.35, 50.67, 29.93, 29.71, 28.75, 28.56, 28.32, 28.10, 27.49, 25.78, 25.35. API-ES (m/z): 754.3 [M + Na]+.
Conjugate 50: 1H NMR (400 MHz, DMSO-d6): δ 8.54 (m, 2H), 7.91 (s, 1H), 7.62 (m, 4H), 5.35 (s, 2H), 4.43 (m, 2H), 4.28 (t, 2H, J = 4.4 Hz), 4.00 (t, 2H, J = 4.4 Hz), 3.94 (s, 1H), 2.53~2.57 (m, 1H), 2.39~2.44 (m, 1H), 2.02~2.07 (m, 2H), 1.72~1.77 (m, 2H), 1.53~1.55 (m, 2H), 1.43 (m, 8H), 1.21~1.24 (m, 2H). 13C NMR (100 MHz, DMSO-d6): δ 172.10, 168.19, 144.36, 141.98, 141.15, 135.87, 131.81, 129.19, 125.24, 124.02, 120.82, 119.53, 119.27, 119.03, 113.45, 103.12, 97.95, 75.19, 64.14, 61.36, 51.68, 29.71, 29.04, 28.83, 28.78, 28.62, 28.36, 28.08, 27.38, 25.78, 25.35. API-ES (m/z): 768.3 [M + Na]+.

2.2. Insecticidal Activity of Nine Conjugates against P. xylostella

Bioassay of insecticidal activity of nine conjugates against P. xylostellaas was described previously [15,16]. The insecticidal activity of compounds 42–50 and chlorfenapyragainst P. xylostella larvae was determined by mixing them with diet. Nine conjugates were dissolved in methanol and acetone (10/90, v/v) at three concentration levels (0.5, 1, and 2 mM). Each test solution (1 mL) and artificial diet (1 g) were placed in Petri dishes (6 cm in diameter). An artificial diet (1 g) containing methanol and acetone (1 mL, 10/90, v/v) was used as control. Ten P. xylostella larvae were introduced into each dish. P. xylostella was cultured at 26 °C, 70% relative humidity, and 16:8 h photoperiod (light/dark). The experiments were repeated three times. The mortality of P. xylostella was measured 24 h after culture.

2.3. Sap Collection from Ricinus communis L. Seedlings and Analysis

Castor bean seeds (R. communis L.) were obtained from the Agricultural Science Academy of Zibo, Shandong, China, and grown as previously described [19]. Six days after sowing, average-sized seedlings were selected for the experiments. Phloem sap was collected from the upper part of the hypocotyl according to previously described methods [19,20]. The endosperm of seedlings was carefully removed without bending or crushing the cotyledons. These latter organs were incubated in a buffer solution containing 20 mM MES (pH 5.0), 0.25 mM MgCl2, and 0.5 mM CaCl2 supplemented with 200 μM 42–45 or 200 μM chlorfenapyr. After 1 h of incubation, the hypocotyl was severed in the hook region for phloem exudation, and the collected sap was stored in ice until analysis.
The phloem sap was analyzed by an HPLC (HP 1260 system, Agilent Technologies, Santa Clara, CA, USA, equipped with a quaternary pump, autosampler, and UV–visible detector)after dilution with methanol (phloem sap/methanol, 1/2 or 1/3, v/v). Separations were performed with a C18 reversed-phase column (5 μm, 250 × 4.6 mm i. d, Agilent Co., Santa Clara, CA, USA) at 25 °C. The mobile solvent system consisted of acetonitrile and water containing 0.1% trifluoroacetic acid (80/20, v/v for 42–45 and chlorfenapyr) at a flow rate of 0.5 mL/min. The detector wavelength was 205 nm for 42–45 and chlorfenapyr. External calibration was used to determine their concentrations. A series of standard solutions (0.5, 1, 5, 10, 25, and 50 μM) for linearities was prepared in methanol. Their linear equations were as follows: y = 7.4216x + 2.2817 (R2 = 0.9996) for 42, y = 12.959x + 3.7681 (R2 = 0.9998) for 43, y = 3.0498x + 1.5868 (R2 = 0.9922) for 44, y = 5.1043x + 5.6425 (R2 = 0.9917) for 45, and y = 30.993x + 25.683 (R2 = 0. 9989) for chlorfenapyr.

2.4. Metabolism of Conjugates 42 and 43 in P. xylostella

The metabolism of conjugates 42 and 43 in P. xylostella as was previously described [21,22,23]. The in vivo mechanism of compounds 42 and 43 in P. xylostella larvae was determined by mixing them with diet. The preparation of the artificial diet with 0.5 mM of tested conjugates was mentioned above. The experiments were repeated three times. After 24 h, the P. xylostella were removed, washed with normal saline, added to 0.1 mL methanol, and homogenized in an ice bath. After ultrasonic extraction for 10 min, the worms were centrifuged at 8000 r and 4 °C for 10 min. The supernatant was taken and stored at −20 °C for measurement.
The supernatant was analyzed by LC–MS/MS. Chromatographic conditions: metal straight pipe, flow rate 0.3 mL/min; column temperature 40 °C; injection volume 3 μL; the mobile phase was B methanol 100%. Mass spectrum conditions: Pola: ESI-; acquisition time: 1 min; acquisition model: MS resolution: 45,000 (LE); acquisition rang: 50~1500 Da; collision energy 6 eV; Lockspary: 0.6 ng/mL, 10 uL/min, ESI-m/z: 554.2615, 236.1035. A series of standard solutions (0.125, 0.0625, 0.03125, 0.015625, 0.0078125 and 0.00390625 mg/L) for linearities was prepared in methanol. Tralopyril linear equation was y = 57.7547 × (R2 = 0.9844) (Over Zero Point).

2.5. Physicochemical Properties

The physicochemical properties (molecular mass (MW), the octanol: water partition coefficient (logP), acid dissociation constant polar (pKa), number of hydrogen bond donors (HBDs), and number of hydrogen bond acceptors (HBAs)) of the conjugates 42–45, tralopyril and chlorfenapyr were predicted using ACD/Percepta 14.0.0 software.

3. Results and Discussion

3.1. Synthesis and Characterization

The main conjugating method employed between glutamic acid moieties and tralopyril was click chemistry.Terminal alkyne was introduced into tralopyril to afford 4, which reacted with azide intermediates 14–22 (compounds 14–22 were selected and prepared according to the literature) to obtain intermediates 23–31 via click reaction (shown in Scheme 1, Scheme 2 and Scheme 3), and the yields reached 80%. Scheme 4 shows that intermediates 23–31 were coupled with purchased Boc-Glu-OH to afford compounds 33–41 under the condition that EDC and DMAP were used together. Scheme 5 shows that the protecting groups on glutamic acidtralopyril conjugates 33–41 were taken off in the presence of TFA in CH2Cl2 to produce final conjugates 42–50 as white solids or liquid in high yields.
The structures of compounds 4, 23–31, 33–41, and 42–50 were confirmed by 1H, 13C NMR, and API-ES spectra. Signals of terminal alkyne could be observed in the 1H and 13C NMR spectra for compound 4. The characteristic signals of 1-, 2-, and 3-triazole could be observed in the 1H and 13C NMR spectra of click reaction products 23–31. Compounds 33–41 were further confirmed by the signals of the six methyl groups. The removal of six methyl groups was confirmed by the 1H and 13C NMR spectra of final conjugates 42–50. The mass spectra of the 28 compounds exhibited excellent correlation with the calculated molecular mass.

3.2. Insecticidal Activity

The insecticidal activity of conjugates 42–50 against P. xylostella was preliminarily evaluated, and conjugates 42, 43, 44, and 45 exhibited preferable activity under the tested concentrations (Table 1). According to the analysis of the linker structure–activity relationship of the nine conjugates, a longer linker chain showed a lower insecticidal activity. Based on the preliminary activity, the insecticidal activity of conjugates 42, 43, 44, and 45 against P. xylostella was further evaluated and compared with that of chlorfenapyr over a wide range of concentrations. Table 2 shows that conjugate 42 showed the highest insecticidal activity, which may be due to the different abilities of the mixed-function oxidases to act on the conjugates of different carbon chain lengths, of which 42 was the highest. Previous studies showed that specific promoiety introduction that confers phloem mobility to pesticides usually reduced direct biological activity [2,4,5]. This phenomenon was also observed in the present work, and the 24 h LC50 value of these new conjugates was higher than that of tralopyril and chlorfenapyr. Hsu et al. described the strategy of phloem-mobile propesticide to overcome this inherent incompatibility [2]. Conjugates 42–45 shared the same O-methylene group on pyrrole N with chlorfenapyr. Thus, conjugates 42, 43, 44, and 45 may have a proinsecticidal mechanism similar to that of chlorfenapyr. This inference needs to be confirmed by obtaining direct evidence in further experiments.

3.3. Phloem Mobility of Conjugates

Chlorfenapyr was selected as the control in the phloem systemicity test because tralopyril has an intolerable phytotoxicity to plants. Conjugates 42–45 were selected for further test because of their insecticidal activity. The phloem sap of conjugates 42–45 or chlorfenapyr was collected within 2 h for detection. When the cotyledons were incubated in a chlorfenapyr solution at the same concentration, it was not detected in the phloem sap (Table 3). Upon observation, their concentrations in the phloem sap were approximately 24.84, 41.88, 36.77, and 30.58 μM. The detected concentrations of conjugates 43–45 were remarkably higher than that of conjugate 42, and conjugate 43 had the best phloem mobility.
The physicochemical properties of conjugates 42–45 were calculated to predict transmembrane behavior in plants (Table 4). The “Rule of Five” can be used to predict the diffusion of endogenous molecules or xenobiotics through plant membranes [24,25]. According to this rule, poor absorption, or permeation of small molecules is more probable when their MW, logP, HBDs, and HBAs are more than 500 D, 5, 5, and 10, respectively; moreover, at least three in four parameters must satisfy this range [26]. Considering that the two parameters (MW and HBAs) of conjugates 42–45 in the table are out of range, they would be expected to diffuse through the membranes poorly. This result suggests the existence of a carrier-mediated absorption and transport mechanism other than passive diffusion, which is worthy of further study.
Linker length is very important for the transporter recognition of carrier-medicated conjugates [8,27]. Based on the results of the phloem systemicity test, the linker arm with the three-atom unit was the optimal length for uptake and transport of glutamic acid–tralopyril conjugates. Such an optimum in phloem mobility may be due to the structural requirement for the recognition by transporters. A shorter linker may lead to the interaction of amino acid moiety and tralopyril and affect the molecular recognition by transporters.

3.4. Metabolism of Conjugates 42 and 43 in P. xylostella

Conjugates 42 and 43 were selected to study the in vivo mechanism in P. xylostella and investigate the pro-insecticide properties of conjugates. Figure 2 shows that tralopyril was detected in all the P. xylostella treated with conjugates 42 and 43, and the tralopyril concentrations were 0.5950 and 0.3172 nmol/kg, respectively, which is consistent with the experimental results of insecticidal activity (Table 2). The results suggest that the different linker lengths influenced the release of the active parent compound in P. xylostella. This behavior may involve the enzymatic bioactivation to release tralopyril because chlorfenapyr is released upon metabolism by P450s in vivo [28,29].

4. Conclusions

In this study, nine conjugates containing tralopyril and glutamic acid were synthesized via click chemistry with good overall yield. The results concerning insecticidal activity against P. xylostella indicated that conjugates 42, 43, 44, and 45 exhibited preferable activity under the tested concentrations. The metabolism of conjugates 42 and 43 in P. xylostella showed that they can release the active compound tralopyril in vivo. The results concerning phloem mobility demonstrated that the phloem mobilities of conjugates 43, 44, and 45 were higher than that of conjugate 42. Although the conjugates exhibited direct insecticidal activity that was lower than that of chlorfenapyr, conjugates 42 and 43 exhibited proinsecticide behavior. Such conjugates can achieve the primary transport of proinsecticide to the plant phloem. Then, the proinsecticide releases the active compound tralopyril at the desired site after being eaten by insects. The appropriate structural design of the linkers may enable controlling the bioactivation and phloem mobility of glutamic acid–tralopyril conjugates. This study expanded on the site-vectorized insecticide delivery strategy by investigating the effect of linker length on insecticidal activity and phloem mobility. As a promising phloem-mobile proinsecticide, the systemic insecticidal activity and phloem loading mechanism of conjugates 42 and 43 will be further investigatedin other plants.

Author Contributions

T.X.L., Y.C. and W.Y. conceived, designed andanalyzed the data; T.X.L., H.F.L. and C.Y.M. performed the experiments; Y.C. and W.Y. contributed reagents/materials/analysis tools; T.X.L. and W.Y. wrote the paper. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31760528) and the Independent Innovation Project of Guizhou academy of agricultural sciences (Grant No. [2014]005 and [2014]009).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

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Molecules 26 04570 g001 550
Figure 1. Chemical structure of tralopyril and chlorfenapyr.
Figure 1. Chemical structure of tralopyril and chlorfenapyr.
Molecules 26 04570 g001
Molecules 26 04570 sch001 550
Scheme 1. Synthetic routes of compound 4. Reagents and conditions: THF, Bromochloromethane (5 equiv), NaH, Propargyl alcohol, 65 °C, 48 h.
Scheme 1. Synthetic routes of compound 4. Reagents and conditions: THF, Bromochloromethane (5 equiv), NaH, Propargyl alcohol, 65 °C, 48 h.
Molecules 26 04570 sch001
Molecules 26 04570 sch002 550
Scheme 2. Synthetic routes of compounds 14–22. Reagents and conditions: NaN3, n-Bu4NBr, 110 °C, 15 h.
Scheme 2. Synthetic routes of compounds 14–22. Reagents and conditions: NaN3, n-Bu4NBr, 110 °C, 15 h.
Molecules 26 04570 sch002
Molecules 26 04570 sch003 550
Scheme 3. Synthetic routes of compounds 23–31. Reagents and conditions: CuSO4·5H2O (0.4 equiv), sodium ascorbate (0.8 equiv), DMF, 60 °C, 3 h.
Scheme 3. Synthetic routes of compounds 23–31. Reagents and conditions: CuSO4·5H2O (0.4 equiv), sodium ascorbate (0.8 equiv), DMF, 60 °C, 3 h.
Molecules 26 04570 sch003
Molecules 26 04570 sch004 550
Scheme 4. Synthetic routes of compounds 33–41. Reagents and conditions: CH2Cl2, EDC, DMAP, rt, 6 h.
Scheme 4. Synthetic routes of compounds 33–41. Reagents and conditions: CH2Cl2, EDC, DMAP, rt, 6 h.
Molecules 26 04570 sch004
Molecules 26 04570 sch005 550
Scheme 5. Synthetic routes of compounds 42–50. Reagents and conditions: CH2Cl2, TFA, rt, 12 h.
Scheme 5. Synthetic routes of compounds 42–50. Reagents and conditions: CH2Cl2, TFA, rt, 12 h.
Molecules 26 04570 sch005
Molecules 26 04570 g002 550
Figure 2. Metabolism of conjugates 42 and 43 in P. xylostella.
Figure 2. Metabolism of conjugates 42 and 43 in P. xylostella.
Molecules 26 04570 g002
Table
Table 1. Mortality percentage of P. xylostella larvae after 24 h of exposure to the diet containing the conjugates 42–50.
Table 1. Mortality percentage of P. xylostella larvae after 24 h of exposure to the diet containing the conjugates 42–50.
Concentration
2 mM1 mM0.5 mM0.1 mM
42100.00 ± 0.00 a100.0 ± 0.00 a100.00 ± 0.00 a43.33 ± 0.15 b
43100.00 ± 0.00 a100.00 ± 0.00 a76.66 ± 0.15 b0.00 ± 0.00 c
44100.00 ± 0.00 a100.00 ± 0.00 a73.33 ± 0.12 b0.00 ± 0.00 c
45100.00 ± 0.00 a100.00 ± 0.00 a60.00 ± 0.26 c0.00 ± 0.00 c
46100.00 ± 0.00 a83.33 ± 0.12 b48.33 ± 0.19 c0.00 ± 0.00 c
47100.00 ± 0.00 a23.33 ± 0.12 c3.33 ± 0.06 d0.00 ± 0.00 c
48100.00 ± 0.00 a3.33 ± 0.058 d0.00 ± 0.00 d0.00 ± 0.00 c
49100.00 ± 0.00 a0.00 ± 0.00 d0.00 ± 0.00 d0.00 ± 0.00 c
50100.00 ± 0.00 a0.00 ± 0.00 d0.00 ± 0.00 d0.00 ± 0.00 c
Chlorfenapyr100.00 ± 0.00 a100.00 ± 0.00 a100.00 ± 0.00 a100.00 ± 0.00 a
Note: English letters of a, b, c and d indicate the difference in 5% level.
Table
Table 2. The insecticidal activity ofconjugates 42, 43, 44, and 45 against P. xylostella.
Table 2. The insecticidal activity ofconjugates 42, 43, 44, and 45 against P. xylostella.
LC50SE95% Confidence Limits
420.2397 mM0.03660.1644 mM–0.3494 mM
430.4413 mM0.06470.3311 mM–0.5882 mM
440.4400 mM0.06240.3309 mM–0.5849 mM
450.4602 mM0.06550.3479 mM–0.6087 mM
Chlorfenapyr0.0249 mM0.01450.0079 mM–0.0784 mM
Tralopyril0.0224 mM0.06010.0133 mM–0.0381 mM
Note: LC50 indicate the median lethal concentration; SE is the abbreviation of Standard error.
Table
Table 3. Phloem mobility of 42–45 and chlorfenapyr. The cotyledons were incubated in a buffered solution containing 200 μM 42–45 and chlorfenapyr at pH 5.5. The sap was collected within two hour of incubation.
Table 3. Phloem mobility of 42–45 and chlorfenapyr. The cotyledons were incubated in a buffered solution containing 200 μM 42–45 and chlorfenapyr at pH 5.5. The sap was collected within two hour of incubation.
Concentration in Phloem Sap (μM)
4224.8493 ± 0.6730 d
4341.887 ± 0.4591 a
4436.7447 ± 0.6832 b
4530.5837 ± 1.9420 c
chlorfenapyr0.00 e
Note: English letters of a, b, c, d and e indicate the difference in 5% level.
Table
Table 4. Predicted physicochemical descriptor MW, logP, pKa, HBD, HBA of tralopyril, chlorfenapyr, 42, 43, 44, and 45 a.
Table 4. Predicted physicochemical descriptor MW, logP, pKa, HBD, HBA of tralopyril, chlorfenapyr, 42, 43, 44, and 45 a.
FeatureMW (D)logPpKaHBDHBA
Tralopyril349.534.0214 b12
Chlorfenapyr407.614.761403
42633.813.003.91311
43647.833.223.92311
44661.863.523.92311
45675.883.763.92311
a All parameters were computed using ACD/Percepta 14.0.0 software. b Assumed nonacidic and assigned pKa of 14.
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Li, T.X.; Chen, Y.; Liu, H.F.; Ma, C.Y.; Yang, W. Vectorizing Pro-Insecticide: Influence of Linker Length on Insecticidal Activity and Phloem Mobility of New Tralopyril Derivatives. Molecules 2021, 26, 4570. https://doi.org/10.3390/molecules26154570

AMA Style

Li TX, Chen Y, Liu HF, Ma CY, Yang W. Vectorizing Pro-Insecticide: Influence of Linker Length on Insecticidal Activity and Phloem Mobility of New Tralopyril Derivatives. Molecules. 2021; 26(15):4570. https://doi.org/10.3390/molecules26154570

Chicago/Turabian Style

Li, Tian Xing, Yao Chen, Hui Fang Liu, Chi Yu Ma, and Wen Yang. 2021. "Vectorizing Pro-Insecticide: Influence of Linker Length on Insecticidal Activity and Phloem Mobility of New Tralopyril Derivatives" Molecules 26, no. 15: 4570. https://doi.org/10.3390/molecules26154570

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