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Profiling and Structural Characterization of High Neu5Gc or Sulfate-Containing O-glycans from Hyla Rabbit Intestinal Mucin

1
Key Laboratory of Marine Drugs, Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
2
Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(7), 1365; https://doi.org/10.3390/molecules24071365
Received: 4 February 2019 / Revised: 1 April 2019 / Accepted: 4 April 2019 / Published: 7 April 2019
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Abstract

Intestinal mucins constitute the major component of the mucus covering the epithelium of the gastrointestinal tract, thereby forming a barrier against microbial colonization. Rabbits are bred in large numbers worldwide, with little known about intestinal O-glycosylation despite this insight being crucial to the understanding of host-pathogen interactions. In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and the O-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches. Thirty-three O-glycans were identified, and most of them were sulfated or sialylated glycans. It was worth noting that Neu5Gc-containing structures within sialylated O-glycans accounted for 91%, which were extremely different from that of other species including humans, mice, chickens, etc. Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin. These structural characterization reflected species diversity and may provide deeper insights into explaining the adaptability of hyla rabbit to the environment. View Full-Text
Keywords: Hyla rabbit intestinal mucin; O-glycan; Neu5Gc; structural characterization Hyla rabbit intestinal mucin; O-glycan; Neu5Gc; structural characterization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Fu, Q.; Li, G.; Wang, C.; Wang, Y.; Li, Q.; Hao, J.; Yu, G. Profiling and Structural Characterization of High Neu5Gc or Sulfate-Containing O-glycans from Hyla Rabbit Intestinal Mucin. Molecules 2019, 24, 1365.

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