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Open AccessArticle

UHPLC-HR-MS/MS-Guided Recovery of Bioactive Flavonol Compounds from Greco di Tufo Vine Leaves

1
Department of Environmental Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100 Caserta, Italy
2
Istituto di Scienze dell’Alimentazione, Consiglio Nazionale delle Ricerche (CNR), 83100 Avellino, Italy
3
Dipartimento di Scienze e Tecnologie, via De Sanctis, snc, 82100 Benevento, Italy
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(19), 3630; https://doi.org/10.3390/molecules24193630
Received: 20 September 2019 / Revised: 2 October 2019 / Accepted: 6 October 2019 / Published: 8 October 2019
(This article belongs to the Special Issue Food Bioactives: Chemical Challenges and Bio-Opportunities)
Leaves of Vitis vinifera cv. Greco di Tufo, a precious waste made in the Campania Region (Italy), after vintage harvest, underwent reduction, lyophilization, and ultrasound-assisted maceration in ethanol. The alcoholic extract, as evidenced by a preliminary UHPLC-HR-MS analysis, showed a high metabolic complexity. Thus, the extract was fractionated, obtaining, among others, a fraction enriched in flavonol glycosides and glycuronides. Myricetin, quercetin, kaempferol, and isorhamnetin derivatives were tentatively identified based on their relative retention time and TOF-MS2 data. As the localization of saccharidic moiety in glycuronide compounds proved to be difficult due to the lack of well-established fragmentation pattern and/or the absence of characteristic key fragments, to obtain useful MS information and to eliminate matrix effect redundancies, the isolation of the most abundant extract’s compound was achieved. HR-MS/MS spectra of the compound, quercetin-3-O-glucuronide, allowed us to thoroughly rationalize its fragmentation pattern, and to unravel the main differences between MS/MS behavior of flavonol glycosides and glycuronides. Furthermore, cytotoxicity assessment on the (poly)phenol rich fraction and the pure isolated compound was carried out using central nervous system cell lines. The chemoprotective effect of both the (poly)phenol fraction and quercetin-3-O-glucuronide was evaluated. View Full-Text
Keywords: food waste recovery; grape leaves; UHPLC-HR-MS/MS analysis; flavonol glycuronides recovery food waste recovery; grape leaves; UHPLC-HR-MS/MS analysis; flavonol glycuronides recovery
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Piccolella, S.; Crescente, G.; Volpe, M.G.; Paolucci, M.; Pacifico, S. UHPLC-HR-MS/MS-Guided Recovery of Bioactive Flavonol Compounds from Greco di Tufo Vine Leaves. Molecules 2019, 24, 3630.

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