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Open AccessArticle

Serum N-Glycosylation in Parkinson’s Disease: A Novel Approach for Potential Alterations

1
Faculty of Materials Science and Engineering, Institute of Chemistry, University of Miskolc, 3515 Miskolc, Hungary
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Faculty of Mechanical Engineering and Information Technology, University of Miskolc, 3515 Miskolc, Hungary
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Characterisation and Comparability Laboratory, NIBRT – The National Institute for Bioprocessing Research and Training, Foster Avenue, Mount Merrion, Blackrock, Co., Dublin A94 X099, Ireland
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School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4 D04 V1W8, Ireland
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(12), 2220; https://doi.org/10.3390/molecules24122220
Received: 15 May 2019 / Revised: 7 June 2019 / Accepted: 12 June 2019 / Published: 13 June 2019
(This article belongs to the Section Analytical Chemistry)
In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson’s disease. Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte. The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing. The annotated structures were quantified in 15 controls and 15 Parkinson’s disease patients by label-free quantitation. Lower sialylation and increased fucosylation were found in Parkinson’s disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids. The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients. View Full-Text
Keywords: glycosylation; Parkinson’s disease; capillary electrophoresis; label-free quantitation; support vector machine glycosylation; Parkinson’s disease; capillary electrophoresis; label-free quantitation; support vector machine
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MDPI and ACS Style

Váradi, C.; Nehéz, K.; Hornyák, O.; Viskolcz, B.; Bones, J. Serum N-Glycosylation in Parkinson’s Disease: A Novel Approach for Potential Alterations. Molecules 2019, 24, 2220.

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