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Molecules 2018, 23(7), 1656;

Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 19 June 2018 / Revised: 3 July 2018 / Accepted: 6 July 2018 / Published: 7 July 2018
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Epimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingredients in different Epimedium are significantly varied, which may derive from their different species, regions and processing methods. In this research, 13 batches of raw Epimedium collected from 6 provinces were identified. After optimization of the processing method of Epimedium, a liquid chromatography–mass spectrometry (LC–MS/MS) method for simultaneous determination of 16 compounds was established to evaluate the quality of raw and processed. Then the multivariate statistical technique was applied to compare different batches of Epimedium based on the LC–MS/MS data. As a conclusion, the herbs collected from 6 areas were ascribed to 5 species by microscopic and appearance features. Meanwhile, all of the raw and processed samples were classified by partial least squares discriminant analysis (PLS-DA) based on the 16 analyzed compounds. The comparison results indicate that processing and species both have important influences on Epimedium compositions contents. View Full-Text
Keywords: Epimedium; identification; processing; LC–MS/MS; comparison Epimedium; identification; processing; LC–MS/MS; comparison

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Zhang, H.; Wang, H.; Wei, J.; Chen, X.; Sun, M.; Ouyang, H.; Hao, J.; Chang, Y.; Dou, Z.; He, J. Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species. Molecules 2018, 23, 1656.

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