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Molecules 2018, 23(5), 991; https://doi.org/10.3390/molecules23050991

Isolation of High Purity Anthocyanin Monomers from Red Cabbage with Recycling Preparative Liquid Chromatography and Their Photostability

1
Xinjiang Production & Construction Group, Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin, Alar 843300, China
2
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
3
Analytic Center, Tarim University, Alar 843300, China
4
Ethnic Medicine Institute, Southwest University for Nationalities, Chengdu 610041, China
*
Authors to whom correspondence should be addressed.
Received: 23 March 2018 / Revised: 18 April 2018 / Accepted: 20 April 2018 / Published: 24 April 2018
(This article belongs to the Special Issue Qualitative and Quantitative Analysis of Bioactive Natural Products)
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Abstract

Anthocyanins from red cabbage are of great importance for their applications in the food industry as natural colorants and their beneficial effects on human wellness as natural antioxidants. This study aimed to develop an effective method for the isolation of anthocyanins with the help of a combination of alternate recycling and direct recycling preparative liquid chromatography. Ten major components of anthocyanins from red cabbage were isolated and their structures were identified by HPLC-MS/MS. Meanwhile, the stability of the isolated anthocyanins under various light conditions was also investigated so as to provide data for their storage. In sum, the results showed that twin column recycling preparative chromatography is an effective method for the isolation of anthocyanin monomers with similar structures. Besides, the stability of various anthocyanins from red cabbage was related to the number of acylated groups and mainly affected by illumination. View Full-Text
Keywords: anthocyanins; red cabbage; recycling preparative high performance liquid chromatography; stability anthocyanins; red cabbage; recycling preparative high performance liquid chromatography; stability
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Chen, Y.; Wang, Z.; Zhang, H.; Liu, Y.; Zhang, S.; Meng, Q.; Liu, W. Isolation of High Purity Anthocyanin Monomers from Red Cabbage with Recycling Preparative Liquid Chromatography and Their Photostability. Molecules 2018, 23, 991.

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