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Molecules 2018, 23(4), 974; https://doi.org/10.3390/molecules23040974

Application of the Response Surface Methodology to Optimize the Fermentation Parameters for Enhanced Docosahexaenoic Acid (DHA) Production by Thraustochytrium sp. ATCC 26185

1,2
,
1,2,* , 1,2
,
2,3
and
1,2,*
1
Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315211, China
2
Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315211, China
3
Ningbo Entry-Exit Inspection and Quarantine Bureau Technology Center, Ningbo 315211, China
*
Authors to whom correspondence should be addressed.
Received: 26 February 2018 / Revised: 13 April 2018 / Accepted: 18 April 2018 / Published: 22 April 2018
(This article belongs to the Section Natural Products Chemistry)
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Abstract

The aim of this study was to determine the cumulative effect of fermentation parameters and enhance the production of docosahexaenoic acid (DHA) by Thraustochytrium sp. ATCC 26185 using response surface methodology (RSM). Among the eight variables screened for effects of fermentation parameters on DHA production by Plackett-Burman design (PBD), the initial pH, inoculum volume, and fermentation volume were found to be most significant. The Box-Behnken design was applied to derive a statistical model for optimizing these three fermentation parameters for DHA production. The optimal parameters for maximum DHA production were initial pH: 6.89, inoculum volume: 4.16%, and fermentation volume: 140.47 mL, respectively. The maximum yield of DHA production was 1.68 g/L, which was in agreement with predicted values. An increase in DHA production was achieved by optimizing the initial pH, fermentation, and inoculum volume parameters. This optimization strategy led to a significant increase in the amount of DHA produced, from 1.16 g/L to 1.68 g/L. Thraustochytrium sp. ATCC 26185 is a promising resource for microbial DHA production due to the high-level yield of DHA that it produces, and the capacity for large-scale fermentation of this organism. View Full-Text
Keywords: Thraustochytrium; docosahexaenoic acid (DHA); Plackett-Burman designs; response surface methodology Thraustochytrium; docosahexaenoic acid (DHA); Plackett-Burman designs; response surface methodology
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Wu, K.; Ding, L.; Zhu, P.; Li, S.; He, S. Application of the Response Surface Methodology to Optimize the Fermentation Parameters for Enhanced Docosahexaenoic Acid (DHA) Production by Thraustochytrium sp. ATCC 26185. Molecules 2018, 23, 974.

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