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Open AccessArticle

Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics

1
Radiation Protection, University Hospital No 2, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, Poland
2
Department of Radiological and Isotopic Diagnostics and Therapy, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, Poland
3
Department of General Physiology, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland
4
Department of Sleep Medicine and Metabolic Disorders, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland
5
Department of Nephrology, Hypertension, and Kidney Transplantation, Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland
6
Department of Clinical Physiology, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(4), 866; https://doi.org/10.3390/molecules23040866
Received: 20 March 2018 / Revised: 3 April 2018 / Accepted: 5 April 2018 / Published: 10 April 2018
(This article belongs to the Special Issue The Antioxidant Capacities of Natural Products)
Oxidative reactions can result in the formation of electronically excited species that undergo radiative decay depending on electronic transition from the excited state to the ground state with subsequent ultra-weak photon emission (UPE). We investigated the UPE from the Fe2+-EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid)–H2O2 system with a multitube luminometer (Peltier-cooled photon counter, spectral range 380 to 630 nm). The UPE of 92.6 µmol/L Fe2+—185.2 µmol/L EGTA—2.6 mmol/L H2O2 reached 4319 ± 755 relative light units during 2 min measurement and was about seven times higher (p < 0.001) than the UPE of incomplete systems (Fe2+-H2O2, EGTA-H2O2) and medium alone. Substitution of Fe2+ with Cr2+, Co2+, Mn2+ or Cu2+ as well as of EGTA with EDTA (ethylenediaminetetraacetic acid) or citrate completely abolished UPE. Experiments with ROS scavengers revealed the dependence of UPE on hydroxyl radicals suggesting occurrence of oxidative attack and cleavage of the ether bond in EGTA backbone structure and formation of triplet excited carbonyl groups with subsequent light emission. Plant phenolics (ferulic, chlorogenic and caffec acids) at concentration 87 µmol/L and ascorbate at 0.46 mmol/L inhibited UPE by 90 ± 4%, 90 ± 5%, 97 ± 2% and 92 ± 1%, respectively. Quenching of UPE from Fe2+-EGTA-H2O2 system can be used for evaluation of antioxidant activity of phytochemicals. View Full-Text
Keywords: chemiluminescence; Fenton system; plant phenolic acids; antioxidant activity chemiluminescence; Fenton system; plant phenolic acids; antioxidant activity
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MDPI and ACS Style

Nowak, M.; Tryniszewski, W.; Sarniak, A.; Wlodarczyk, A.; Nowak, P.J.; Nowak, D. Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics. Molecules 2018, 23, 866. https://doi.org/10.3390/molecules23040866

AMA Style

Nowak M, Tryniszewski W, Sarniak A, Wlodarczyk A, Nowak PJ, Nowak D. Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics. Molecules. 2018; 23(4):866. https://doi.org/10.3390/molecules23040866

Chicago/Turabian Style

Nowak, Michal; Tryniszewski, Wieslaw; Sarniak, Agata; Wlodarczyk, Anna; Nowak, Piotr J.; Nowak, Dariusz. 2018. "Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics" Molecules 23, no. 4: 866. https://doi.org/10.3390/molecules23040866

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