Next Article in Journal
Enhanced Stability of DNA Oligonucleotides with Partially Zwitterionic Backbone Structures in Biological Media
Previous Article in Journal
miR-103/miR-195/miR-15b Regulate SALL4 and Inhibit Proliferation and Migration in Glioma
Previous Article in Special Issue
HPLC-UV/Vis-APCI-MS/MS Determination of Major Carotenoids and Their Bioaccessibility from “Delica” (Cucurbita maxima) and “Violina” (Cucurbita moschata) Pumpkins as Food Traceability Markers
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(11), 2939; https://doi.org/10.3390/molecules23112939

A Novel Method for the Determination of Vancomycin in Serum by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application in Patients with Diabetic Foot Infections

1
Department of Pharmacy, National Cancer Center/National Clinical Research Center for Cancer/Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
2
Institute of Aviation Medicine of Air Force, Beijing 100142, China
*
Authors to whom correspondence should be addressed.
Received: 11 September 2018 / Revised: 6 November 2018 / Accepted: 6 November 2018 / Published: 10 November 2018
Full-Text   |   PDF [1726 KB, uploaded 10 November 2018]   |  

Abstract

A novel, precise, and accurate high-performance liquid chromatography-tandem mass spectrometry (Q-trap-MS) method was developed, optimized, and validated for determination of vancomycin in human serum using norvancomycin as an internal standard. Effect of different parameters on the analysis was evaluated. ZORBAX SB-C18 column (150 × 4.6 mm, 5 μm) using water (containing 0.1% formic acid, v/v)–acetonitrile (containing 0.1% formic acid, v/v) as a mobile phase was chosen. The calibration curve was linear over the concentration ranges of 1 to 2000 ng/mL for vancomycin. The limit of detection (LOD) and limit of quantification (LOQ) for vancomycin were 0.3 and 1.0 ng/mL. Recoveries were between 87.2 and 102.3%, which gave satisfactory precision. A total of 100 serum samples (from 50 patients with diabetic foot proven Gram-positive infection and 50 nondiabetic patients with pneumonia requiring hospitalization and antibiotic therapy) were analyzed by this method. The trough vancomycin concentrations of diabetic foot infection (DFI) patients and nondiabetic patients were 8.20 ± 2.83 μg/mL (range: 4.80–14.2 μg/mL) and 15.80 ± 5.43 μg/mL (range: 8.60–19.5 μg/mL), respectively. The method is sensitive, precise, and reproducible, it could be applied for routine laboratory analysis of vancomycin in serum samples. View Full-Text
Keywords: vancomycin; norvancomycin; high performance liquid chromatography—tandem mass spectrometry; Diabetic Foot Infection (DFI); MRSA; Serum vancomycin; norvancomycin; high performance liquid chromatography—tandem mass spectrometry; Diabetic Foot Infection (DFI); MRSA; Serum
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Liu, M.; Yang, Z.-H.; Li, G.-H. A Novel Method for the Determination of Vancomycin in Serum by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application in Patients with Diabetic Foot Infections. Molecules 2018, 23, 2939.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top