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Modulation of HO-1 by Ferulic Acid Attenuates Adipocyte Differentiation in 3T3-L1 Cells

Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Kyeonggi 13488, Korea
Author to whom correspondence should be addressed.
These authors equally contributed to this work.
Academic Editors: Dong-Kug Choi and Palanivel Ganesan
Molecules 2017, 22(5), 745;
Received: 11 March 2017 / Revised: 28 April 2017 / Accepted: 3 May 2017 / Published: 5 May 2017
(This article belongs to the Special Issue Natural Products and Chronic Diseases)
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Ferulic acid (FA) is phenolic compound found in fruits. Many studies have reported that FA has diverse therapeutic effects against metabolic diseases. However, the mechanism by which FA modulates adipogenesis via the expression of heme oxygenase-1 (HO-1) implicated in suppression of adipocyte differentiation is not fully understood. We investigated whether HO-1 can be activated by FA and suppress adipogenic factors in 3T3-L1. Our results showed that FA suppresses triglyceride-synthesizing enzymes, fatty acid synthase (FASN) and acetyl-CoA carboxylase (ACC). We observed that the expression of CCAAT/enhancer binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ) were suppressed by FA. In addition, HO-1 inhibitor stimulated lipid accumulation, while FA attenuated lipid accumulation in 3T3-L1 treated with HO-1 inhibitor. We also observed that the expression of HO-1 had the same tendency as C/EBP homologous protein 10 (CHOP10) during the mitotic clonal expansion (MCE) of adipogenesis. We next employed siRNA against HO-1 to clarify whether HO-1 regulates CHOP10. The results indicated that CHOP10 is downstream of HO-1. Furthermore, FA-mediated HO-1/CHOP10 axis activation prevented the initiation of MCE. Therefore, we demonstrated that FA is a positive regulator of HO-1 in 3T3-L1, and may be an effective bioactive compound to reduce adipocyte tissue mass. View Full-Text
Keywords: Ferulic acid; 3T3-L1; HO-1; adipogenesis; obesity Ferulic acid; 3T3-L1; HO-1; adipogenesis; obesity

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Koh, E.-J.; Kim, K.-J.; Seo, Y.-J.; Choi, J.; Lee, B.-Y. Modulation of HO-1 by Ferulic Acid Attenuates Adipocyte Differentiation in 3T3-L1 Cells. Molecules 2017, 22, 745.

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