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Open AccessArticle

Chemical Composition, Antioxidant and α-Glucosidase-Inhibiting Activities of the Aqueous and Hydroethanolic Extracts of Vaccinium myrtillus Leaves

1
Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, Croatia
2
Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Bd. 8, 8005-139 Faro, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: László Dux
Molecules 2017, 22(5), 703; https://doi.org/10.3390/molecules22050703
Received: 30 January 2017 / Revised: 25 April 2017 / Accepted: 26 April 2017 / Published: 28 April 2017
(This article belongs to the Special Issue Chemistry and Pharmacology of Modulators of Oxidative Stress)
Vaccinium myrtillus (bilberry) leaf is traditionally used in southeastern Europe for the treatment of diabetes. In the present study, the ability of bilberry leaf extracts to inhibit carbohydrate-hydrolyzing enzymes and restore glutathione concentration in Hep G2 cells subjected to glucose-induced oxidative stress was investigated. A comprehensive analysis of the antioxidant activity of two bilberry leaf extracts was performed. The aqueous extract showed excellent total antioxidant and chelating activity. Its antioxidant activity in the β-carotene-linoleic acid assay was very good, reaching the activity of the antioxidant standard BHA (93.4 ± 2.3% vs. 95.1 ± 2.4%, respectively). The hydroethanolic extract (ethanol/H2O, 8:2, v/v), on the other hand, was a better radical scavenger and Fe2+ reducing agent. Furthermore, the aqueous extract was able to efficiently increase glutathione concentration in Hep G2 cells subjected to glucose-induced oxidative stress and restore it to the levels observed in non-hyperglycaemic cells. The hydroethanolic extract strongly inhibited α-glucosidase, with the IC50 statistically equal to the antidiabetic drug acarbose (0.29 ± 0.02 mg/mL vs. 0.50 ± 0.01 mg/mL, respectively). Phytochemical analysis revealed the presence of quercetin and kaemferol derivatives, as well as chlorogenic and p-coumaric acid. The study results indicate that V. myrtillus leaf may have promising properties as a supporting therapy for diabetes. View Full-Text
Keywords: α-glucosidase inhibition; antioxidant activity; polyphenol; glutathione; Vaccinium myrtillus α-glucosidase inhibition; antioxidant activity; polyphenol; glutathione; Vaccinium myrtillus
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MDPI and ACS Style

Bljajić, K.; Petlevski, R.; Vujić, L.; Čačić, A.; Šoštarić, N.; Jablan, J.; Saraiva de Carvalho, I.; Zovko Končić, M. Chemical Composition, Antioxidant and α-Glucosidase-Inhibiting Activities of the Aqueous and Hydroethanolic Extracts of Vaccinium myrtillus Leaves. Molecules 2017, 22, 703. https://doi.org/10.3390/molecules22050703

AMA Style

Bljajić K, Petlevski R, Vujić L, Čačić A, Šoštarić N, Jablan J, Saraiva de Carvalho I, Zovko Končić M. Chemical Composition, Antioxidant and α-Glucosidase-Inhibiting Activities of the Aqueous and Hydroethanolic Extracts of Vaccinium myrtillus Leaves. Molecules. 2017; 22(5):703. https://doi.org/10.3390/molecules22050703

Chicago/Turabian Style

Bljajić, Kristina; Petlevski, Roberta; Vujić, Lovorka; Čačić, Ana; Šoštarić, Nina; Jablan, Jasna; Saraiva de Carvalho, Isabel; Zovko Končić, Marijana. 2017. "Chemical Composition, Antioxidant and α-Glucosidase-Inhibiting Activities of the Aqueous and Hydroethanolic Extracts of Vaccinium myrtillus Leaves" Molecules 22, no. 5: 703. https://doi.org/10.3390/molecules22050703

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