Highly Efficient Enzymatic Preparation of Daidzein in Deep Eutectic Solvents
AbstractDaidzein, which is scarce in nature, has gained significant attention due to its superior biological activity and bioavailability compared with daidzin. So far, it has been widely used in the medicine and health care products industries. The enzymatic approach for the preparation of daidzein has prevailed, benefitted by its high efficiency and eco-friendly nature. Our present research aimed at providing a preparation method of daidzein by enzymatic hydrolysis of daidzin in a new “green” reaction medium-deep eutectic solvents (DESs). Herein, the DESs were screened via evaluating enzyme activity, enzyme stability and the substrate solubility, and the DES (ChCl/EG 2:1, 30 vol %) was believed to be the most appropriate co-solvent to improve the bioconversion efficiency. Based on the yield of daidzein, response surface methodology (RSM) was employed to model and optimize the reaction parameters. Under these optimum process conditions, the maximum yield of 97.53% was achieved and the purity of daidzein crude product reached more than 70%, which is more efficient than conversions in DESs-free buffer. Importantly, it has been shown that DESs medium could be reused for six batches of the process with a final conversion of above 50%. The results indicated that this procedure could be considered a mild, environmentally friendly, highly efficient approach to the economical production of daidzein, with a simple operation process and without any harmful reagents being involved. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 958 KB)
Share & Cite This Article
Cheng, Q.-B.; Zhang, L.-W. Highly Efficient Enzymatic Preparation of Daidzein in Deep Eutectic Solvents. Molecules 2017, 22, 186.
Cheng Q-B, Zhang L-W. Highly Efficient Enzymatic Preparation of Daidzein in Deep Eutectic Solvents. Molecules. 2017; 22(1):186.Chicago/Turabian Style
Cheng, Qi-Bin; Zhang, Li-Wei. 2017. "Highly Efficient Enzymatic Preparation of Daidzein in Deep Eutectic Solvents." Molecules 22, no. 1: 186.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.